FastQCFastQC Report
Thu 28 Sept 2023
SRR6128380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6128380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124755766
Total Bases12.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT29720472.382292294209472No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG22921051.8372737978299136No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA14029941.1245925098163398No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC13070411.0476798322892746Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT11607930.9304523848621152No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC8514000.6824534266416191No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC7423490.5950418355813711No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC7160840.5739887004501258No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACAC6613960.5301526504193802No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCT5938930.4760445300780727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC5760550.4617461929575263Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC5627700.4510973865528588No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG5135100.41161223762595467TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA5093460.40827451614541005No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC4946800.39651874687699806No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC4726300.3788442131003388No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACG4616100.3700109540427975No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4604560.36908594669684447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT4405660.3531427958207559Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA4049510.32459501711528105Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA3876160.3106998677720435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT3698390.2964504261871151No Hit
GGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTCC3565980.2858368886933851Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT3558150.28520926239192823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3321690.26625542902762506Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG3271420.2622259559530098No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC3086410.24739618046992715No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT2855160.22885996307377088No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT2844620.22801511234358499Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG2728480.21870572298838675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAAGATCGGAAGAGCACAC2640380.2116439251392998No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2560500.20524101467181885No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT2501490.20051097277539862Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTAGATCGGAAGAGCACACGTCTG2494960.19998755007443902Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA2490640.1996412734943249Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTGA2402840.19260352262996805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC2373280.19023409306789074No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA2304390.18471210380769093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC2288890.18346967626329994Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC2250870.18042212173183242Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG2210580.17719261168257344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2203570.17663071380604564Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGATAAAGTGCTGATAGATTTATAAGATCGGAAGAGCACACGTCTGAAC2171050.17402402066129752Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2163040.17338196616900256Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG2146490.17205537417805603No Hit
TAGCTAATTAACACAATCCCGGGGCGTTCTATAGATCGGAAGAGCACACG2142750.17175558843508684No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2055890.16479318478955113No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC2008260.1609753251805612No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCGAGATCGGAAGAGCACACGTC1957590.15691378945963907No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1922030.1540634202029588No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1903780.1526005619652081No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1839110.1474168336235457Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC1837260.14726854388437646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCTTGGACTACATATGGTTGAGGGTTGTAGATCGGAAGAGCACACGTCT1782780.14290161145738145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC1767910.14170968258092376No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1766780.14161910560510685No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC1750130.14028449795258363No Hit
GGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCTG1684510.13502462082594244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC1672000.13402186156269522No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC1651820.13240430105651388No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT1651010.1323393741977425No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1611720.12919002076425068No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAAGAGCACACG1603870.12856079133047846No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG1555250.12466357667187902No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC1526350.12234705047620806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG1469070.11775567952506501Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACGTCTGAACTC1443610.11571489208763305Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1422820.11404843604583374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT1376740.11035481919128291No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTAGATCGGAAGAGCACACGTCT1358540.10889596878432056Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1311640.10513662350484065Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT1303850.10451220346801444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAAC1283950.10291708681424794Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)