FastQCFastQC Report
Fri 29 Sept 2023
SRR6128382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6128382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131661431
Total Bases13.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT19322851.4676165869714723No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT16271661.235871422360585Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG15060981.1439173861022367No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC13565611.030340464702985Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC13322431.0118703631589725No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT8771340.6662042128343569No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT7839550.5954325378705628Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT7596860.5769996529963282No Hit
GGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTCC6240290.47396492295454395Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC5808800.44119222735775976No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC5806960.44105247496512473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG5628360.4274873785930521Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT5386660.4091296865822459Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC5336380.40531080054871954No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC5323660.4043446861822427Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA5192020.3943463139178549No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5088750.38650271088121474No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA4878890.3705633428820928No Hit
TCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTG4451490.3381012925493723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTGA4443180.3374701282108957Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4399130.33412442555025856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTAGATCGGAAGAGCACACGTCTG4066750.3088793710589398Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCTG3981970.30244012766350686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGATAAAGTGCTGATAGATTTATAAGATCGGAAGAGCACACGTCTGAAC3440380.2613050742248123Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT3237630.245905727699405No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG3171740.24090122490010002TruSeq Adapter, Index 12 (100% over 49bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC3099760.23543417206212802No Hit
AAACGACAATGGATGTGATGCCAATAGATCGGAAGAGCACACGTCTGAAC3059630.23238620275971328Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCT3002930.22807970239971034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCT2988200.22696092373475724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTC2975590.22600316413088356No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2935540.22296127101945293No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC2927230.2223301066809763No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC2917280.221574380427325No Hit
CCGGAAGGCCCGGGTTCGATTCCCGGTATGGGAAGATCGGAAGAGCACAC2786770.2116618343605881No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCT2682610.2037506336992494Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC2577830.19579234255778366No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC2535050.19254309942901957No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC2494500.18946323012393812No Hit
TAAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTG2418660.18370300107098184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2359450.1792058602188518Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT2332760.17717869100177105Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT2297990.17453782649529306No Hit
ATAAACGACAATGGATGTGATGCCAATAGATCGGAAGAGCACACGTCTGA2269770.1723944501256408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2260860.17171771435478322Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAACGACAATGGATGTGATGCCAGATCGGAAGAGCACACGTCTGAACT2240270.17015385470024247Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTGA2218650.16851176408678106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC2191460.16644661867604948Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAAACGACAATGGATGTGATGCCAATGAGATCGGAAGAGCACACGTCTG2162680.1642607089695083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2151830.16343662556728553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2149700.16327484698233305No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2126240.16149300397623662Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGACAATGGATGTGATGCCAATGAGATCGGAAGAGCACACGTCTGAA2111600.1603810610261406Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2087920.1585825084948378No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAAGAGCACACG2086760.1584944037255679No Hit
GTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCACACGTC2035760.1546208319731843No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA2006060.15236504607032564No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1999650.15187819126772215No Hit
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAGATCGGAAGAGCACACG1957490.14867603861908502No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1943550.14761726234010017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1923190.1460708717346388No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA1911420.14517691213609854Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC1748030.13276705157488378No Hit
TTGATAAAGTGCTGATAGATTTATATGAGATCGGAAGAGCACACGTCTGA1720710.130692032353803Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1661510.12619565102554597No Hit
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC1649130.1252553604707517No Hit
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT1645930.12501231283138645Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1619950.12303906980928986Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT1605270.12192408876370181No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1544710.11732441218871455Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1528700.11610841446801533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG1450140.11014159492159857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGACAATGGATGTGATGCCAGATCGGAAGAGCACACGTCTGAACTCC1421050.10793213997499389Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1397900.10617384220896096No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1397160.10611763744235775No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTAGATCGGAAGAGCACACGTCT1395290.10597560647810367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATAAACGACAATGGATGTGATGCCAATAGATCGGAAGAGCACACGTCTG1392120.10573483741035748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1380270.10483480162083306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT1375380.10446339444692805No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1368350.10392944916419752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACACGT1344090.10208684424825977No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC1337460.1015832799204499No Hit
TGTTTCCTTACTTACTTGATTAAATGGAAGATCGGAAGAGCACACGTCTG1323020.10048652744781425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1318110.1001136012261632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)