FastQCFastQC Report
Fri 29 Sept 2023
SRR6128384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6128384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153749621
Total Bases15.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG44489442.8936292467348586TruSeq Adapter, Index 6 (100% over 49bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA33680202.1905875137084077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG32783312.1322530609685213No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA31300622.03581770130022Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC27577751.7936792182401542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA25389391.6513465096606645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT23484351.5274411635785432No Hit
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA19608031.2753221681112306Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG19534581.2705449205627635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC18296201.1899996813650682Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT16091211.0465853441030597Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT15808451.0281944044597027Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT14837120.9650183137687214No Hit
GGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTCC14516780.9441831404579527Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA11147230.7250248766466878Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC10357180.6736393841257013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC10176830.6619092739096898No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC9047640.5884658408361215No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA9008500.5859201435039635Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT7797350.5071459655825753No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT7791220.5067472654127713No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC7474180.4861267267774273Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC7466370.48561875804558896Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC7247270.47136831641360594No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC7124730.4633982154661702No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT6728380.43761929013145345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG6577160.427783818732145No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT6428490.4181142014002103No Hit
GTTCAGTGGTAGAATGCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA6373610.4145447617070874Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCT6243040.4060523830494515Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT5622400.3656854542750385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC5616620.36530951838899167No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA5444230.3540971330264287Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACG5284040.3436782455548297No Hit
GGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGTCTGA5080710.3304534975081337Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC5076010.3301478056976804No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA5019530.3264743007073819Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4550010.2959363392512038No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT4196340.27293335571864596Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC4111990.26744716333317015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC4099140.2666113889152286No Hit
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC4066270.2644734974663775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC4054020.26367674753487685Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACAC3961820.25767998478513326No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT3792830.24668873817906842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT3781960.24598174456638175Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3659920.23804416402431328No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC3608080.234672448395824No Hit
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG3597180.23396350355881526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA3535460.22994918472026674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTC3532330.229745606982667No Hit
GTCTAGGGGTATGATTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAG3498170.22752381288796802Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3390450.22051761675562112Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG3255420.2117351560821083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG3218100.20930783302548758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA3166540.20595432882400405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT3143730.2044707479311445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT3087940.20084212110025298No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC3081110.20039789236293465Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC3051890.1984973998732654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2980250.19383787619222814Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC2917060.18972794736189952Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC2875960.18705477004070142No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC2798170.1819952453736455No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC2795860.18184500110084825No Hit
GTATAGTGGTTAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC2779620.18078873833451597Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT2769190.18011036267855254Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG2746350.17862483056137096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACGTCTGAACTC2628160.17093765714063125Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC2601440.16919976667779882No Hit
GGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTCTGAACTCCA2601390.1691965146372621Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTGA2521940.1640290222243865Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG2486100.16169795956765318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2473030.16084787617135005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAAGAGCACACG2460200.16001340256962324No Hit
TGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAAC2426190.15780136459653452Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA2375070.15447647835177428Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG2369230.15409664001708334Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCTG2275980.14803158441606826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGGAGATCGGAAGAGCACACGTCTGAACTCCA2261520.1470910942928438Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT2226020.14478214551176033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAACTCCA2192160.1425798636602818Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA2138160.1390676598806055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG2094730.13624293747039545No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTAGATCGGAAGAGCACACGTCTG2044100.13294992122289523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2000490.13011349146675294No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1973290.12834438141476784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAG1949290.12678340195713395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA1941480.12627543322529555No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG1929260.12548063451811697No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT1903070.12377721568497393Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1892170.12306827084796522No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1885200.12261493639714403No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1882970.12246989538920554No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1882610.12244648069734104No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGAGATCGGAAGAGCACACGTCTG1850300.12034501210250138Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1848600.1202344427242523No Hit
CTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTGA1801390.11716386604946492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC1747960.11368873553190743No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT1726160.11227084585788995No Hit
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC1705080.1108997855676015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGGTAGATCGGAAGAGCACACGTCTGAACTCC1657390.10779798930366144Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC1654610.10761717584981884No Hit
AGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAACTC1651540.10741750056086317Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1585670.10313326235776542No Hit
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC1585650.10313196154155073Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCT1584410.10305131093623963Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTG1553820.10106171253586374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)