FastQCFastQC Report
Fri 29 Sept 2023
SRR6128386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6128386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119044734
Total Bases12 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG34035372.8590403671278732No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA26877742.2577848760618004No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT21792101.8305807630264435No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC19401941.6298024572846708Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT13890911.1668647182663283No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC12793731.0746993646942837No Hit
GGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTCC11660100.979472136919555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA10838720.9104745448043086No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACAC10561170.8871597797849672No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT10403440.8739101386878649No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC10170250.8543217039739028Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACG9384790.788341464982399No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC8432590.7083547265517851Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC7959210.6685898428736881No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG6947590.5836117034794668No Hit
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA6381550.5360631911698002Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC6292340.5285693695615298No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC5343390.4488556377470674No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCT4828310.4055878691786568Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACGTCTGAACTC4722430.39669373363461835Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT4705070.3952354582941905Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT4535580.38099795325679836No Hit
GGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGTCTGA4429450.37208281720382524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4104890.34481911648439656No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC4059330.34099198373613065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC3982740.33455826781888565Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC3891220.3268704015080583No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC3567200.29965206188792864No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTGA3548710.2980988642471157Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG3457830.2904647592391613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC3307100.27780313239223164No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG3238370.27202967247589466TruSeq Adapter, Index 6 (100% over 49bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT3233480.27161890252113124Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC3222430.270690680026216No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC3054520.25658589820529143No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA2866140.2407615947127909Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA2865450.24070363330813105Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2633660.2212328014442033Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT2607100.2190017073749772No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2559840.21503177116595512Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAAGAGCACACG2504220.2103595779381556No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA2457630.2064459230930786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG2428710.20401658421950863No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA2417440.20306988127673078Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGATCCTGGGTTCGAATCCCAGCGGTGCCAGATCGGAAGAGCACACG2372340.19928138946490484No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2333800.19604395100752628Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG2308780.19394222007333814No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC2295970.1928661539955224Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAAGATCGGAAGAGCACAC2269080.19060733925450243No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT2244590.18855012939925592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2181060.1832134800687614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACAC2074920.1742975039954308No Hit
GTATAGTGGTTAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC2071080.17397493617819332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC2036720.17108862623020352Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1854970.1558212562346521No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1843170.15483003221293265Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1820570.15293158620523273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCTGAACT1807030.15179419864132757Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1735560.14579057314706587No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1721690.14462546491136685Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1685770.1416081117876243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1671820.14043628338906616No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTC1667350.14006079428931312No Hit
GGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCTG1664770.13984406903710667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACA1626790.13665367171974194No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC1600070.1344091373247976No Hit
GGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTCCA1541490.12948829807121076Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT1500920.1260803354812822No Hit
GGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTC1496760.12573088701260823Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1480930.12440113478686089No Hit
AAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTGA1477220.12408948723426944Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT1470410.12351743337088729No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1419800.1192660903421398No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1409870.11843195012725216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1377790.11573716482074714Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC1347920.11322802401322514No Hit
TGGACGAGGCGCCGCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT1342350.11276013267415927Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1336670.11228300111116211No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1335990.11222587972685966No Hit
GTTCAGTGGTAGAATGCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA1315460.11050131793313932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC1298750.10909764391594172No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAAGAGCACA1268710.10657422276234411No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACACGT1240480.10420284529343397No Hit
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC1222200.10266728808012623Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC1205280.10124597363542348Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC1204500.1011804520475471No Hit
CGGGAGACCGGGGTTCAATTCCCCGTCGGGGAAGATCGGAAGAGCACACG1190680.10001954391363503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)