FastQCFastQC Report
Fri 29 Sept 2023
SRR6128388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6128388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150431385
Total Bases15.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC24446811.6251136689328494Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC14182770.9428065825492465No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCT10834010.7201961213080634Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT10808440.718496343033736No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA9629450.6401224053079083No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT8711520.5791025589507137Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGATAAAGTGCTGATAGATTTATAAGATCGGAAGAGCACACGTCTGAAC8254660.548732566678157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTCC7725130.513531800561432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT7699680.5118399993458812Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC7465870.49629736507444905No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT7256120.4823541310877381No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC7226650.48039509840316896Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT7088200.4711915668395927No Hit
AAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTGA6356510.4225521157037808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT6149210.4087717466670935No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC5891920.3916682678950274No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC5810860.3862797646913907No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT5298460.35221772371503457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC5127390.3408457616740017No Hit
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG4910400.3264212451410987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4870520.323770202607654Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGTCTGA4747650.3156023591752479Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT4315560.28687896478517433No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA3936400.26167411807050767Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC3868720.2571750569204691Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT3858920.25652359712037487No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA3663580.24353827494176164Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG3646070.24237428911526673No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG3645030.242305154605869Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3628310.24119368441632044Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT3533820.23491241538459545No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA3506610.2331036173069868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG3438350.22856600037286104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA3360230.22337293510925263Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT3345560.22239773967380547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG3132110.208208546374814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA3123990.20766876539759305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCTG2866560.1905559800569542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC2789950.18546329278295218No Hit
TGTTTCCTTACTTACTTGATTAAATGGAAGATCGGAAGAGCACACGTCTG2785620.1851754539120942Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC2755910.18320046710997176No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2669390.1774490077319969Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC2638330.17538427901863696No Hit
TAAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTG2598160.17271395859314861Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2572680.17102016311290358Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAACT2509430.16681558838270352Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTG2464270.16381355526308555Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT2336550.15532330570512265No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTAGATCGGAAGAGCACACGTCT2334630.15519567276469604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCT2326260.1546392729150237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT2320510.15425703884864186Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCTGAA2282000.1516970677362307Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT2276870.15135604847352832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCT2256850.15002520916762152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC2209520.14687892423512555Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTGA2203680.14649070737466122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC2183240.14513194836303608No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2171770.1443694744949666No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA2169970.14424981861331662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC2169670.14422987596637496No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG2155880.14331317896195664No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTAGATCGGAAGAGCACACGTCTG2130310.14161340068762912Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2105980.13999605202066046No Hit
TTGATAAAGTGCTGATAGATTTATATGAGATCGGAAGAGCACACGTCTGA2096370.13935722256362926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2083580.13850700104901648Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT2056500.13670684478508258Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCTG1974970.13128709810123732Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1967170.13076858928075416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGAAGGTGGCTCGGGGGGGGCGGCGAGATCGGAAGAGCACACGTCTG1957140.13010184011800463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTC1939990.1289617854678397Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTC1918870.1275578231231468No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG1891990.12577096195717402Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGATAAAGTGCTGATAGATTTATAGATCGGAAGAGCACACGTCTGAACT1823590.12122403845447544Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1778070.1181980741585275Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1769200.11760843656395241Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT1733300.1152219664799337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG1706690.11345305369620841Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1677860.11153656532511484No Hit
TTGATAAAGTGCTGATAGATTTATATAGATCGGAAGAGCACACGTCTGAA1667040.11081730052541895Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1625700.10806920377685814No Hit
GGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACACGTCTGA1538140.10224860988948549Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)