FastQCFastQC Report
Fri 29 Sept 2023
SRR6128390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6128390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107288123
Total Bases10.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT21064461.963354322080926No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC19481781.8158375275145786Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG17150901.5985832840043255No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT14458741.3476552292745394Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC13642771.2716011445181121No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT12684481.182281844934504No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC9526780.8879622211304786No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT8782630.8186022603825402No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC7800650.7270748878699276Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT6686710.623247924656115Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT5949440.5545292278065113No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC5749700.5359120692231701No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA5662530.527787218348484No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC5451400.5081084324683358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC5117680.4770034051206209No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG5005620.4665586329625694No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG4994700.46554081293788685TruSeq Adapter, Index 6 (100% over 49bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG4856530.45266240700287025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG4524850.42174752185756853No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAAGAGCACACG4048620.37735957035989903No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA3898160.3633356508623047No Hit
TCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTG3878250.36147990024953647Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGAACTCC3585260.3341711924627482Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAGATCGGAAGAGCACACG3496740.3259205121894061No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT3366740.31380360713366195Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3361530.3133179988618125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC3124780.29125125061606305Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG3002210.2798268732877357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACACGT2877750.26822633480129016No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC2863310.2668804262704829No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT2808050.26172980955217195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC2652730.24725290421941673Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG2635100.2456096654799339No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA2627340.24488637945506792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCTG2606680.2429607236208243Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACG2551850.23785018589615928No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT2467840.23001986902128954No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2455880.22890511375616107Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGGGCAGATCGGAAGAGCACACGTCT2445300.2279189840985474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC2367800.22069544454608458No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC2342440.2183317159905948No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT2298800.21426416417034347No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2129610.19849447827510225No Hit
AAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTGA2095840.1953468791694678Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTGA2055150.1915542878870199Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC2022000.18846447709780514No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1949150.18167434991849005No Hit
TAAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCTG1942450.18104986327330938Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1918480.1788156923949541No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCAGATCGGAAGAGCACACGTCT1902950.1773681882756025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1869010.17420474398643362Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCACACGTC1842300.17171518603228803No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1773940.16534355811220594No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA1753670.16345425299312955Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1725480.1608267487352724No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1695640.15804545299016928No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTC1693910.15788420494596592No Hit
ATAAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGTCT1675910.15620647963055517Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1668820.15554564227020728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC1664390.15513273542869233No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1634910.15238499418989743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAAGATCGGAAGAGCACAC1592600.14844140762906255No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1576020.14689603619964534No Hit
TAGCTAATTAACACAATCCCGGGGCGTTCTAAGATCGGAAGAGCACACGT1512660.14099044308939956No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG1446400.13481454978944873No Hit
CCGGAAGGCCCGGGTTCGATTCCCGGTATGGGAAGATCGGAAGAGCACAC1436600.13390112156216957No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1401800.13065751928570882No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1379940.12862001509710447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC1376480.1282975190086977Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG1303120.12145985627877935No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1301860.1213424155067006No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1290480.12028172027951313Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1215500.11329306227120778No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1198680.11172532117091842No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC1170000.10905214550169733No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAAGATCGGAAGAGCACACGTC1150050.10719266661045045No Hit
CGGGAGACCGGGGTTCAATTCCCCGTCGGGGAAGATCGGAAGAGCACACG1147210.10692795883846343No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAAGAGCACACGT1145300.10674993354110593No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA1144080.10663622104750588No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1117930.10419885899206197No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA1101570.10267399309427754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1095240.10208399302502477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)