FastQCFastQC Report
Tue 4 Jul 2023
SRR6160937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24563352
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG14354025.843673127348417TruSeq Adapter, Index 10 (100% over 36bp)
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA10528194.286137331745277TruSeq Adapter, Index 10 (100% over 40bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT7563443.0791562975606914Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC4050281.6489117609029909TruSeq Adapter, Index 10 (100% over 42bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT3453241.4058504718737086TruSeq Adapter, Index 10 (100% over 41bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT3228911.3145233598411161Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG2850411.160432012699244TruSeq Adapter, Index 10 (100% over 49bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA2566951.0450324532254391TruSeq Adapter, Index 10 (100% over 40bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2403470.9784780187980858Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT1963750.799463363143597TruSeq Adapter, Index 10 (100% over 43bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1519720.6186940609734372Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA1389170.5655457773027068No Hit
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA1332520.5424829640514861Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1272240.5179423394657211Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG1189150.48411552299539573TruSeq Adapter, Index 10 (100% over 36bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG1173910.4779111580536728Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA995980.40547397602737606TruSeq Adapter, Index 10 (100% over 40bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA873330.3555418657844418TruSeq Adapter, Index 10 (100% over 40bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT830290.33801982726136076TruSeq Adapter, Index 10 (100% over 39bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT811180.33023994445057825TruSeq Adapter, Index 10 (100% over 41bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT777520.31653660298480435TruSeq Adapter, Index 10 (100% over 39bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT761190.30988848753215764Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG755410.30753538849258033TruSeq Adapter, Index 10 (100% over 36bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG745330.30343171404293684Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT714150.29073800676715456TruSeq Adapter, Index 10 (100% over 48bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT699970.284965179019541TruSeq Adapter, Index 10 (100% over 39bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT665290.270846584782077Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC649160.2642798914415264TruSeq Adapter, Index 10 (100% over 42bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG643410.26193900571876344TruSeq Adapter, Index 10 (100% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC630750.25678498602308025TruSeq Adapter, Index 10 (100% over 50bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA620170.25247775629319646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA617960.25157804195453454TruSeq Adapter, Index 10 (100% over 35bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG578890.23567223235656112TruSeq Adapter, Index 10 (100% over 36bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT574920.2340560034314535TruSeq Adapter, Index 10 (100% over 48bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT570310.23217922374763836TruSeq Adapter, Index 10 (100% over 43bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC530190.21584594806116036No Hit
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG496380.2020815400113144No Hit
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC488640.19893050427319528TruSeq Adapter, Index 10 (100% over 44bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA482900.19659368965603716No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA481020.1958283218023338TruSeq Adapter, Index 10 (100% over 47bp)
AGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA479970.19540085571382926TruSeq Adapter, Index 10 (100% over 35bp)
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT469090.19097149281580136TruSeq Adapter, Index 10 (100% over 38bp)
CTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC444000.1807570888533454TruSeq Adapter, Index 10 (100% over 44bp)
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT440480.17932405968045403Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC438510.17852205187630743Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT438450.1784976252426786Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT427480.17403162239420744Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT425320.17315226358356953TruSeq Adapter, Index 10 (100% over 43bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC394520.16061325832076992TruSeq Adapter, Index 10 (100% over 42bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC392080.15961990855319746TruSeq Adapter, Index 10 (100% over 42bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT378990.15429083131650762TruSeq Adapter, Index 10 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATT373220.1519418033825351TruSeq Adapter, Index 10 (97% over 48bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG372330.15157947498370744TruSeq Adapter, Index 10 (100% over 36bp)
GGGCCGGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG371340.15117643552883173TruSeq Adapter, Index 10 (100% over 36bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC370120.1506797606450455TruSeq Adapter, Index 10 (100% over 42bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC367270.14951949554767607TruSeq Adapter, Index 10 (100% over 42bp)
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT364730.1484854347240556Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA350110.14253347832983054TruSeq Adapter, Index 10 (100% over 40bp)
CGATAGACTTGGGGTGTACGCGTCGAGGCAGATCGGAAGAGCACACGTCT344340.14018445039585803Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344090.14008267275573788No Hit
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA336990.1371921877763263TruSeq Adapter, Index 10 (100% over 40bp)
TAAACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGC334190.13605227820698088No Hit
GGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT332210.13524619929722945TruSeq Adapter, Index 10 (100% over 41bp)
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA325510.13251855854201006TruSeq Adapter, Index 10 (100% over 40bp)
CGATAGACTTGGGGTGTACGCGTCGAGGCAACAGATCGGAAGAGCACACG324860.13225393667769772No Hit
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT317450.12923724742453715Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA315540.1284596662540194TruSeq Adapter, Index 10 (100% over 47bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTG313760.1277350094563641No Hit
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT311630.1268678639625406TruSeq Adapter, Index 10 (100% over 41bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTAGATCGGAAGAGCA307810.12531270162150507No Hit
ATAAGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA305250.12427049858667498Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT300850.12247921212056073No Hit
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC295150.12015868192582185Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTG288950.1176345964508427No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT286530.11664938889447986TruSeq Adapter, Index 10 (100% over 48bp)
TCCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT286220.11652318462073091TruSeq Adapter, Index 10 (100% over 41bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA277810.11309938480708985Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCTCGTAGATCAGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT276910.11273298530265739Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT268430.10928068774978268TruSeq Adapter, Index 10 (100% over 43bp)
GTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTGGATTAGATC267080.108731088493134No Hit
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT266700.1085763864801514TruSeq Adapter, Index 10 (100% over 38bp)
TTAAGGCGGCCATAGCGGTGGGGTTACTCCCGTAGATCGGAAGAGCACAC266560.10851939100168413No Hit
GGGAAACTGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG257880.10498567133671333TruSeq Adapter, Index 10 (100% over 36bp)
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA257090.10466405399393373TruSeq Adapter, Index 10 (100% over 40bp)
GGGCTCGTAGATCAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT255040.10382947734494868Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGAAAGCTCAGTAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTC251980.10258371902987834Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT249810.10170028911363563TruSeq Adapter, Index 10 (100% over 48bp)
ACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT246510.10035682426404996TruSeq Adapter, Index 10 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)