FastQCFastQC Report
Tue 4 Jul 2023
SRR6160938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31943259
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT20260516.342655894941715TruSeq Adapter, Index 9 (100% over 36bp)
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA11808573.6967330102416915TruSeq Adapter, Index 9 (100% over 40bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT8004642.5058933404384316Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT5075321.5888547877973251TruSeq Adapter, Index 9 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4177971.3079347977612428TruSeq Adapter, Index 9 (100% over 49bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC3967441.2420273084847104TruSeq Adapter, Index 9 (100% over 42bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT3788711.1860749712482375Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2929440.9170761192525785Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA2771350.8675852391892762TruSeq Adapter, Index 9 (100% over 40bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT2345860.734383426562706TruSeq Adapter, Index 9 (100% over 43bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT2190980.6858974533562777TruSeq Adapter, Index 9 (100% over 36bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA1861160.582645621725698No Hit
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1844170.577326815651465Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA1550390.48535748966628606TruSeq Adapter, Index 9 (100% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC1409880.4413701181836205TruSeq Adapter, Index 9 (100% over 50bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA1395020.43671811946301403Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT1303730.4081393197857488TruSeq Adapter, Index 9 (100% over 36bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1299120.4066961357950358Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA1248960.3909932921997721TruSeq Adapter, Index 9 (100% over 40bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT1133100.3547227288236307TruSeq Adapter, Index 9 (100% over 48bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG1120910.3509065872082745Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT1119440.3504463962177435TruSeq Adapter, Index 9 (100% over 43bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1065980.3337104708070019Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT929720.2910535834806336TruSeq Adapter, Index 9 (100% over 41bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT900050.2817652387942007Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG898130.28116417301064994TruSeq Adapter, Index 9 (100% over 39bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT890260.278700429408283TruSeq Adapter, Index 9 (100% over 48bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG882510.27627425241738796TruSeq Adapter, Index 9 (100% over 39bp)
CTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC846010.2648477414280115TruSeq Adapter, Index 9 (100% over 44bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA836490.2618674569179056TruSeq Adapter, Index 9 (100% over 47bp)
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA771740.24159713947784728TruSeq Adapter, Index 9 (100% over 38bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC739680.23156059311293192TruSeq Adapter, Index 9 (100% over 42bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG718040.22478608084416182TruSeq Adapter, Index 9 (100% over 39bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT717620.2246545977040101TruSeq Adapter, Index 9 (100% over 36bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG714310.22361838533757625Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC713910.2234931632993365No Hit
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT685960.21474327337733448Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC677800.21218874379724373TruSeq Adapter, Index 9 (100% over 42bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA663600.20774336143973288Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT646830.20249342748653165TruSeq Adapter, Index 9 (100% over 41bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA621810.19466078899463576TruSeq Adapter, Index 9 (100% over 35bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA595870.18654013981478848No Hit
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC593690.18585767970638187TruSeq Adapter, Index 9 (100% over 44bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC590100.1847338119131802TruSeq Adapter, Index 9 (100% over 42bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT574960.1799941577658059TruSeq Adapter, Index 9 (100% over 43bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG570550.17861358479421277Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA561140.17566773634462282TruSeq Adapter, Index 9 (100% over 47bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT554750.17366731428374294TruSeq Adapter, Index 9 (100% over 36bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC537590.16829528884325798TruSeq Adapter, Index 9 (100% over 42bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG523050.16374346775324333No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT519670.16268534153011752TruSeq Adapter, Index 9 (100% over 48bp)
ACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT490070.15341891070037658TruSeq Adapter, Index 9 (100% over 46bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC489470.15323107764301697Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT489220.1531528138691171TruSeq Adapter, Index 9 (100% over 48bp)
TACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG478050.1496559884512723TruSeq Adapter, Index 9 (100% over 45bp)
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA470840.14739886121200094TruSeq Adapter, Index 9 (100% over 40bp)
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA449680.14077461538911856TruSeq Adapter, Index 9 (100% over 40bp)
GGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT448780.14049286580307915TruSeq Adapter, Index 9 (100% over 41bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTG448170.14030190219476352No Hit
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT447010.1399387582838683TruSeq Adapter, Index 9 (100% over 41bp)
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT444270.13908098732192603Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCTCGTAGATCAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT443890.1389620263855983Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAAACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGC432700.13545893986584148No Hit
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT430400.13473891314596298Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT420870.1317554980849011TruSeq Adapter, Index 9 (100% over 36bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC419860.13143931243834575TruSeq Adapter, Index 9 (100% over 42bp)
GGGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT411860.12893487167355092TruSeq Adapter, Index 9 (100% over 41bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT410480.12850285564162378TruSeq Adapter, Index 9 (100% over 43bp)
GGGCTCGTAGATCAGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT408020.12773274010644936Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA397230.12435487562493233TruSeq Adapter, Index 9 (100% over 35bp)
ATTCCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA390830.12235132301309645TruSeq Adapter, Index 9 (100% over 40bp)
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT386090.12086744185995549Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA382660.11979366288204969TruSeq Adapter, Index 9 (100% over 40bp)
GGGCTCGTAGATCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG377980.11832856503464471Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCTCGTAGATCAGCGGTAGATCAGATCGGAAGAGCACACGTCTGAACT377760.11825969291361285Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGATAGACTTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA368720.11542967484939468TruSeq Adapter, Index 9 (100% over 35bp)
GGGCCGGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT366090.11460633994796836TruSeq Adapter, Index 9 (100% over 36bp)
AGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTG365800.11451555397024454No Hit
CGATAGACTTGGGGTGTACGCGTCGAGGCAGATCGGAAGAGCACACGTCT365630.11446233460399265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTAGATCGGAAGAGCA365380.11438407083009282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362740.11355760537771054No Hit
GAAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA360270.11278435929158012TruSeq Adapter, Index 9 (100% over 40bp)
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA351100.10991364406493401TruSeq Adapter, Index 9 (100% over 38bp)
GGGAAACTGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT349580.109437800319623TruSeq Adapter, Index 9 (100% over 36bp)
GGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC349300.10935014489285516TruSeq Adapter, Index 9 (100% over 42bp)
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC348850.10920927009983547Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT347670.10883986508702821TruSeq Adapter, Index 9 (100% over 41bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA347470.10877725406790834TruSeq Adapter, Index 9 (100% over 47bp)
AAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC340010.10644186305473716TruSeq Adapter, Index 9 (100% over 42bp)
GCCGCCTTAGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT338620.10600671647185406TruSeq Adapter, Index 9 (100% over 36bp)
GGGAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT337290.1055903531947069TruSeq Adapter, Index 9 (100% over 41bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA333960.10454787972636105Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTGGATTAGATC333100.1042786523441456No Hit
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA326000.10205596116539016TruSeq Adapter, Index 9 (100% over 40bp)
CGATAGACTTGGGGTGTACGCGTCGAGGCAACAGATCGGAAGAGCACACG323320.1012169735091839No Hit
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT322610.10099470439130834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)