FastQCFastQC Report
Tue 4 Jul 2023
SRR6160939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9974124
Total Bases758 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT9801749.827168781940149TruSeq Adapter, Index 12 (100% over 36bp)
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA5497095.511351172293426TruSeq Adapter, Index 12 (100% over 40bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC5195105.208577715697138TruSeq Adapter, Index 12 (100% over 42bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT3607023.616377739037534Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1767001.7715841511495145Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1424321.428015131955448TruSeq Adapter, Index 12 (100% over 43bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA980140.9826827899873714No Hit
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA907190.9095435348507798TruSeq Adapter, Index 12 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG876770.8790446158479683TruSeq Adapter, Index 12 (100% over 49bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT676430.6781848711726464TruSeq Adapter, Index 12 (100% over 36bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA657720.659426331575585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC641420.6430840442729607Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT600260.6018172623480519TruSeq Adapter, Index 12 (100% over 36bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT516790.5181307150382329TruSeq Adapter, Index 12 (100% over 41bp)
CCGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC516770.5181106631519721TruSeq Adapter, Index 12 (100% over 42bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT489810.4910807204722941TruSeq Adapter, Index 12 (100% over 41bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA479100.4803429353795882Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT478480.4797213269055006Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT476050.4772850227248027No Hit
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC456050.457233136463914Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC453470.4546464431362594TruSeq Adapter, Index 12 (100% over 42bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG443840.44499145990164146Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA437560.4386951676157224TruSeq Adapter, Index 12 (100% over 40bp)
TCCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT409370.4104320339309998TruSeq Adapter, Index 12 (100% over 41bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA353510.35442711560433776TruSeq Adapter, Index 12 (100% over 40bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC343360.34425078332693676No Hit
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT341540.3424260616771959TruSeq Adapter, Index 12 (100% over 36bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG335370.3362400547657118No Hit
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT310650.31145592334725336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT307460.30825764748864165Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC306890.30768616873020627TruSeq Adapter, Index 12 (100% over 50bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC288190.2889376550762754TruSeq Adapter, Index 12 (100% over 42bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA261370.2620480756004237TruSeq Adapter, Index 12 (100% over 39bp)
GGGCTCGTAGATCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG246910.2475505618338011Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC246580.24721970571049645Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTAGATCGGAAGAGCACACGTCT243710.24434226003205894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC239490.24011131203101146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG230440.2310378334979593Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA230280.23087741840787224TruSeq Adapter, Index 12 (100% over 39bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA229900.23049643256891533TruSeq Adapter, Index 12 (100% over 40bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA226430.22701743030265112TruSeq Adapter, Index 12 (100% over 39bp)
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT204280.20480996626871692TruSeq Adapter, Index 12 (100% over 38bp)
AGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTG194410.19491436039896837No Hit
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC193070.19357088401948883Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT191440.1919366552892264TruSeq Adapter, Index 12 (100% over 48bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT184620.18509896207426338Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT179920.1803867688029545TruSeq Adapter, Index 12 (100% over 36bp)
AGGAACTACGAACGGGTGCCACTGGTGTATCGGTTGTCCGAGAGGGCATG178110.1785720730963441No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA171140.1715839907344244TruSeq Adapter, Index 12 (100% over 47bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT167910.16834561110329088TruSeq Adapter, Index 12 (100% over 36bp)
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT167610.16804483280937754Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGAGATCGGAAGAGCACACGTCTG167570.16800472903685576Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCGTAGATCAGCGGTAGATCAGATCGGAAGAGCACACGTCTGAACT164050.16447559705493936Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGAAAGCTCAGTAACACGTGGCCAAGCTACCCTGTGGACGGGAATACT161750.16216963013493715No Hit
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA160310.16072589432415318No Hit
TTAAGGCGGCCATAGCGGTGGGGTTACTCCCGTAGATCGGAAGAGCACAC149770.15015855026466485No Hit
GGGCTCGTAGATCAGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT143270.14364168722987603Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT139320.1396814396933505TruSeq Adapter, Index 12 (100% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137980.13833796331387097No Hit
CTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC136760.13711479825195677TruSeq Adapter, Index 12 (100% over 44bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT136400.13675386429926076TruSeq Adapter, Index 12 (100% over 48bp)
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC136330.13668368269734765Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTG134090.13443787143612812No Hit
AGGAACTACGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT131130.13147019226951662TruSeq Adapter, Index 12 (100% over 35bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGATCGGAAGAGCACACGTCTGAA130750.1310892064305597Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT129640.12997632674308038TruSeq Adapter, Index 12 (100% over 35bp)
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT128260.12859274659107908Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA126370.1266978433394251Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCGATTAGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTA126180.12650735041994665No Hit
GCGCTGGTGGTCTAGTGGTAGGACATGAGCAGATCGGAAGAGCACACGTC122270.12258720665594292No Hit
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC113200.1134936762366299TruSeq Adapter, Index 12 (100% over 44bp)
TAAACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGC109780.11006480368601795No Hit
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC107520.10779894053853752TruSeq Adapter, Index 12 (100% over 42bp)
CGATTAGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACG106200.10647551604531887No Hit
GTCCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA104010.10427983449975155TruSeq Adapter, Index 12 (100% over 40bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTAGATCGGAAGAGCA102590.10285615057522846No Hit
GCGCCGGTAGTCTAGTGGTAGGACCTGAGCAGATCGGAAGAGCACACGTC101790.10205407512479292No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA101530.10179340060340136TruSeq Adapter, Index 12 (100% over 47bp)
CGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT100010.10026945724757381TruSeq Adapter, Index 12 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)