FastQCFastQC Report
Tue 4 Jul 2023
SRR6160940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21546550
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC13807606.408264896236289TruSeq Adapter, Index 11 (100% over 36bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT10364094.810092567023491Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA7744593.594352692194342TruSeq Adapter, Index 11 (100% over 40bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC6027172.7972784506104227TruSeq Adapter, Index 11 (100% over 42bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT4720572.1908704641810406Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT2882371.337740844822025TruSeq Adapter, Index 11 (100% over 43bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT2560721.1884594053340325Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT2348051.089756828819463TruSeq Adapter, Index 11 (100% over 41bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA2083420.9669390227205747TruSeq Adapter, Index 11 (100% over 40bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1907560.8853203877186836Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1897080.8804564999965191TruSeq Adapter, Index 11 (100% over 49bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA1338330.6211342419087974No Hit
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC1295400.6012099384820307TruSeq Adapter, Index 11 (100% over 36bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1145910.5318299217276083Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA1064580.49408373962420893TruSeq Adapter, Index 11 (100% over 40bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA938180.4354200556469597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT933270.4331412685557549Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA889760.4129477805031432Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC827170.38389904648307965Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT820780.3809333744845462TruSeq Adapter, Index 11 (100% over 41bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC804610.37342869276055796TruSeq Adapter, Index 11 (100% over 36bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA775390.3598673569550578TruSeq Adapter, Index 11 (100% over 40bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC721350.3347867756090882TruSeq Adapter, Index 11 (100% over 42bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG716580.33257296411722526Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC664170.30824888439216486TruSeq Adapter, Index 11 (100% over 42bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC626390.290714754798332TruSeq Adapter, Index 11 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC607400.2819012788590285TruSeq Adapter, Index 11 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT589240.27347301540153757TruSeq Adapter, Index 11 (100% over 48bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC582840.2705027022887655TruSeq Adapter, Index 11 (100% over 36bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC580440.26938883487147597TruSeq Adapter, Index 11 (100% over 36bp)
GGGCTCGTAGATCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG572530.2657177135086592Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC571660.2653139365698917TruSeq Adapter, Index 11 (100% over 42bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC553530.25689959645511695TruSeq Adapter, Index 11 (100% over 39bp)
GGGCTCGTAGATCAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT528640.24534786311497664Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG515350.2391798222917358Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT512960.23807059598868496TruSeq Adapter, Index 11 (100% over 48bp)
GGGCTCGTAGATCAGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT501470.23273795572841127Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT494330.22942420016197487No Hit
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC487040.22604082788195792TruSeq Adapter, Index 11 (100% over 39bp)
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT485290.2252286328901843Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG480950.22321438931058568Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT465350.21597425109820367TruSeq Adapter, Index 11 (100% over 41bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC448840.20831177149009933No Hit
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC412730.19155270797413043TruSeq Adapter, Index 11 (100% over 44bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT401430.18630824888439215TruSeq Adapter, Index 11 (100% over 43bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA388820.18045580382938337TruSeq Adapter, Index 11 (100% over 47bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT373520.17335489904416254TruSeq Adapter, Index 11 (100% over 43bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT366250.16998080899262297TruSeq Adapter, Index 11 (100% over 43bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG359310.16675987571096068Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCCGGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC355260.16488022444428457TruSeq Adapter, Index 11 (100% over 36bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG346040.16060111711619723No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA339140.1573987482914898TruSeq Adapter, Index 11 (100% over 47bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA317870.14752709830576125TruSeq Adapter, Index 11 (100% over 40bp)
CTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC306180.1421016357607134TruSeq Adapter, Index 11 (100% over 44bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC305060.14158183096597832Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC304250.1412059007126431TruSeq Adapter, Index 11 (100% over 36bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC302150.14023126672251474TruSeq Adapter, Index 11 (100% over 42bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGAGATCGGAAGAGCACACGTCTG297830.13822630537139355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG297150.1379107096031615TruSeq Adapter, Index 11 (100% over 35bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA296270.13750229155015536No Hit
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA294770.1368061244143494TruSeq Adapter, Index 11 (100% over 38bp)
GGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT293760.13633737187624004TruSeq Adapter, Index 11 (100% over 41bp)
GGGCTCGTAGATCAGCGGTAGATCAGATCGGAAGAGCACACGTCTGAACT291980.1355112535417503Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTAGATCGGAAGAGCACACGTCT291620.13534417342915686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT282320.1310279371871599Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAAGGCGGCCATAGCGGTGGGGTTACTCCCGTAGATCGGAAGAGCACAC276070.1281272407879684No Hit
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT271170.12585309481100224Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA265640.12328655863699757TruSeq Adapter, Index 11 (100% over 40bp)
AGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTG263140.12212628007732096No Hit
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC261030.12114700497295391TruSeq Adapter, Index 11 (100% over 42bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC259560.12046476117986407TruSeq Adapter, Index 11 (100% over 42bp)
GGGCTCGTAGATCAGTGGTAGATCATCCCCAGATCGGAAGAGCACACGTC259160.1202791166103158No Hit
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC255010.11835305420125264Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT250960.11647340293457653TruSeq Adapter, Index 11 (100% over 48bp)
GCCGGTGTAGCTCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG250770.1163852217640411Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT248290.11523422543284192TruSeq Adapter, Index 11 (100% over 48bp)
AGGAACTACGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG244740.11358662987810114TruSeq Adapter, Index 11 (100% over 35bp)
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT237430.11019397536960673TruSeq Adapter, Index 11 (100% over 41bp)
GGGCTCGTAGATCAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC234130.10866240767083361Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA232330.10782700710786645TruSeq Adapter, Index 11 (100% over 40bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT232310.10781772487938905TruSeq Adapter, Index 11 (100% over 41bp)
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA230730.10708442882967342TruSeq Adapter, Index 11 (100% over 40bp)
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA230440.10694983651675094TruSeq Adapter, Index 11 (100% over 38bp)
GCCGCCTTAGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC222880.10344115415228888TruSeq Adapter, Index 11 (100% over 36bp)
CCGCCCTTAGCTCAGACTGGTAGAGCGAGATCGGAAGAGCACACGTCTGA222510.10326943292545675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA217050.10073538455112303Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)