FastQCFastQC Report
Tue 4 Jul 2023
SRR6160951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17474431
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA6808253.896121138364963TruSeq Adapter, Index 7 (100% over 40bp)
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG5640403.2278018093979712TruSeq Adapter, Index 7 (100% over 36bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT4227752.419392082065505Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC3931072.2496125911052554TruSeq Adapter, Index 7 (100% over 42bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT3527182.018480601743198TruSeq Adapter, Index 7 (100% over 41bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA3133771.7933459464288135TruSeq Adapter, Index 7 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2445261.399335978378924TruSeq Adapter, Index 7 (100% over 49bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2169411.2414767611031226Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1768641.0121302376025865Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT1623000.9287856068103161TruSeq Adapter, Index 7 (100% over 43bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC1006950.5762419388648478TruSeq Adapter, Index 7 (100% over 39bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC986210.5643731690033283TruSeq Adapter, Index 7 (100% over 39bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA947960.5424840442587229Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC863710.49427074335067045Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC859260.4917241654392066Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT828230.47396679182286394Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG797420.4563353164403465Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA710760.4067428576072091No Hit
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA665030.3805731929125475TruSeq Adapter, Index 7 (100% over 40bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA591810.33867197163673024TruSeq Adapter, Index 7 (100% over 40bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT566470.32417078415886613TruSeq Adapter, Index 7 (100% over 43bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC541710.3100015102065412TruSeq Adapter, Index 7 (100% over 42bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT516650.29566055684445464TruSeq Adapter, Index 7 (100% over 41bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT513230.29370341157317226TruSeq Adapter, Index 7 (100% over 48bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA490890.2809190181929243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG478670.27392594356863464Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC477050.27299887475592194TruSeq Adapter, Index 7 (100% over 42bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC461550.26412877191823875TruSeq Adapter, Index 7 (100% over 39bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT459700.2630700822247088TruSeq Adapter, Index 7 (100% over 48bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC432090.2472698538796485TruSeq Adapter, Index 7 (100% over 50bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC410700.23502911196364562TruSeq Adapter, Index 7 (100% over 42bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC410370.23484026461290786Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA395040.22606744677408952TruSeq Adapter, Index 7 (100% over 40bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT393980.22546084619293186Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG392420.22456811326217146TruSeq Adapter, Index 7 (100% over 36bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC391580.22408741091483894TruSeq Adapter, Index 7 (100% over 42bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA379240.21702566452664468TruSeq Adapter, Index 7 (100% over 35bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT370400.21196684458566922TruSeq Adapter, Index 7 (100% over 41bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA362520.2074573987559309TruSeq Adapter, Index 7 (100% over 40bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT362160.20725138346421695No Hit
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT350550.2006073903064426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA344390.1970822397593375TruSeq Adapter, Index 7 (100% over 40bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG344050.19688766976160768TruSeq Adapter, Index 7 (100% over 36bp)
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA342890.19622384271052945TruSeq Adapter, Index 7 (100% over 40bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG340980.19513081713504718TruSeq Adapter, Index 7 (100% over 36bp)
GCCGGTGTAGCTCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG328620.1880576254528688Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC315320.1804465049534374TruSeq Adapter, Index 7 (100% over 42bp)
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC313850.17960527584560548Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT309840.17731049440179195TruSeq Adapter, Index 7 (100% over 43bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC308090.17630903117818258No Hit
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC300380.17189687034730916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC296070.169430409493734TruSeq Adapter, Index 7 (100% over 44bp)
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT290700.16635734805900118TruSeq Adapter, Index 7 (100% over 38bp)
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT287670.1646233860204089Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA282230.1615102660567317TruSeq Adapter, Index 7 (100% over 47bp)
GGGCTCGTAGATCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG277660.15889501638136316Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAACTACGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA276110.15800800609759483TruSeq Adapter, Index 7 (100% over 35bp)
GGGCCGGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG274010.15680625022926356TruSeq Adapter, Index 7 (100% over 36bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT271800.15554154524401967Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG263990.15107215794322573No Hit
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT259140.1482966741520797TruSeq Adapter, Index 7 (100% over 38bp)
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT257000.1470720276957802Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC253230.14491458977977595TruSeq Adapter, Index 7 (100% over 42bp)
TTAAGGCGGCCATAGCGGTGGGGTTACTCCCGTAGATCGGAAGAGCACAC253150.14486880860383952No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG247810.14181291511008284No Hit
GGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC244740.14005606248352237TruSeq Adapter, Index 7 (100% over 42bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG243920.13958680543017396TruSeq Adapter, Index 7 (100% over 36bp)
GCGGAAAGCTCAGTAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTC243450.13931784102154743Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA235540.13479122725083295TruSeq Adapter, Index 7 (100% over 47bp)
TCCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT234190.1340186699069057TruSeq Adapter, Index 7 (100% over 41bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGAGATCGGAAGAGCACACGTCTG232680.1331545502111056Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT231190.13230187580928957TruSeq Adapter, Index 7 (100% over 41bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT230080.13166666199317162TruSeq Adapter, Index 7 (100% over 43bp)
CCGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC226040.12935471260838194TruSeq Adapter, Index 7 (100% over 42bp)
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT219270.12548048059476158TruSeq Adapter, Index 7 (100% over 41bp)
GGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT218190.12486243471961976TruSeq Adapter, Index 7 (100% over 43bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG217160.12427300207943823TruSeq Adapter, Index 7 (100% over 36bp)
CTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC212250.12146318240633988TruSeq Adapter, Index 7 (100% over 44bp)
GGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT209880.12010691506922314TruSeq Adapter, Index 7 (100% over 41bp)
CAGAACAAAGGGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC207840.11893949508284418Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCGGTTGATCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC205900.11782930156638577Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT205510.11760611833369566TruSeq Adapter, Index 7 (100% over 41bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA204580.11707391216343467Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT200200.11456739278091516TruSeq Adapter, Index 7 (100% over 48bp)
GGGAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT196230.11229550192506985TruSeq Adapter, Index 7 (100% over 41bp)
GAAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA193310.11062448900339016TruSeq Adapter, Index 7 (100% over 40bp)
AGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT191780.10974892401360593TruSeq Adapter, Index 7 (100% over 43bp)
AAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC189970.10871312490804422TruSeq Adapter, Index 7 (100% over 42bp)
GAAGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA189750.10858722667421902TruSeq Adapter, Index 7 (100% over 40bp)
AGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA185910.10638973022927042TruSeq Adapter, Index 7 (100% over 35bp)
GGGAAACTGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG183380.10494190054028082TruSeq Adapter, Index 7 (100% over 36bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA182990.10471871730759073No Hit
CGATAGACTTGGGGTGTACGCGTCGAGGCAACAGATCGGAAGAGCACACG180710.10341395379340249No Hit
GTACGAAAGTGCGATAAGGGGACTCCGAGTAGATCGGAAGAGCACACGTC177970.10184594851757978No Hit
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTAGATCGGAAGAGCA177910.10181161263562745No Hit
GAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC176720.10113061764357306TruSeq Adapter, Index 7 (100% over 42bp)
AAGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT174920.1001005411850034TruSeq Adapter, Index 7 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)