FastQCFastQC Report
Tue 4 Jul 2023
SRR6160952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17025028
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA7497264.403669703215759TruSeq Adapter, Index 8 (100% over 40bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT6488273.8110186955345973Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC5958753.499994243768645TruSeq Adapter, Index 8 (100% over 42bp)
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT5116763.0054341173477073TruSeq Adapter, Index 8 (100% over 36bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT2840381.6683555527779454TruSeq Adapter, Index 8 (100% over 43bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT2702371.58729254365984TruSeq Adapter, Index 8 (100% over 41bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA2488491.4616657311811765TruSeq Adapter, Index 8 (100% over 40bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT2035971.195868811493291Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1893161.111986423752137Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1645160.9663185282279712Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1286430.7556110920933581TruSeq Adapter, Index 8 (100% over 49bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA1137420.6680870069641002TruSeq Adapter, Index 8 (100% over 40bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA1061000.6232001498029841No Hit
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA787910.46279512726792577TruSeq Adapter, Index 8 (100% over 39bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA726250.42657785937268355TruSeq Adapter, Index 8 (100% over 39bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA692320.40664837673101034TruSeq Adapter, Index 8 (100% over 40bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA682320.4007746712663263Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC674380.3961109491273671TruSeq Adapter, Index 8 (100% over 42bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT668620.39272769477970904TruSeq Adapter, Index 8 (100% over 41bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT659310.3872592749920881Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC625580.3674472664597086Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC600860.3529274665510095TruSeq Adapter, Index 8 (100% over 42bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG519130.3049216717881463Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT499880.29361478876862934TruSeq Adapter, Index 8 (100% over 48bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA485820.2853563588852835TruSeq Adapter, Index 8 (100% over 39bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT481230.2826603280769935TruSeq Adapter, Index 8 (100% over 48bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC473940.2783783967932388TruSeq Adapter, Index 8 (100% over 44bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT465300.2733035152717517Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT423780.24891589018238325TruSeq Adapter, Index 8 (100% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC411560.24173822210453924TruSeq Adapter, Index 8 (100% over 50bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT399260.2345135643829778TruSeq Adapter, Index 8 (100% over 43bp)
TCCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT393350.2310422044533495TruSeq Adapter, Index 8 (100% over 41bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT387670.22770593974940895No Hit
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT382640.2247514659006728TruSeq Adapter, Index 8 (100% over 43bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT382530.2246868551405613TruSeq Adapter, Index 8 (100% over 36bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC379480.22289537497383263TruSeq Adapter, Index 8 (100% over 42bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT378260.22217878290714116TruSeq Adapter, Index 8 (100% over 43bp)
GGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT376630.22122136891639765TruSeq Adapter, Index 8 (100% over 41bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT376480.2211332633344274TruSeq Adapter, Index 8 (100% over 36bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG372970.21907159271632326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCCGGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT372440.21876028632669503TruSeq Adapter, Index 8 (100% over 36bp)
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT360120.2115238811942042TruSeq Adapter, Index 8 (100% over 41bp)
CCGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC357890.21021404487557965TruSeq Adapter, Index 8 (100% over 42bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA353640.20771772005308892Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA350500.20587337653717808Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA349740.20542697492186207TruSeq Adapter, Index 8 (100% over 47bp)
GGGCTCGTAGATCAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT340750.20014651370911107Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA331550.1947427046816017Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC325510.19119498658093248No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA318930.18733008838517035TruSeq Adapter, Index 8 (100% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATT312460.18352980094951973TruSeq Adapter, Index 8 (97% over 48bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG310510.18238442838390634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG309600.18184992118662008No Hit
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA297080.17449604194483556TruSeq Adapter, Index 8 (100% over 40bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC295340.17347401719398053Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG291890.1714475888086645TruSeq Adapter, Index 8 (100% over 38bp)
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA287210.16869869465119233TruSeq Adapter, Index 8 (100% over 40bp)
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA286030.16800559740635962TruSeq Adapter, Index 8 (100% over 40bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT279890.16439914225104357TruSeq Adapter, Index 8 (100% over 41bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC275170.16162675327171266TruSeq Adapter, Index 8 (100% over 42bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT274420.16118622536186136TruSeq Adapter, Index 8 (100% over 36bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC268340.15761501243933343TruSeq Adapter, Index 5 (100% over 35bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT267800.1572978323442405TruSeq Adapter, Index 8 (100% over 36bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC264580.15540649918461222TruSeq Adapter, Index 8 (100% over 42bp)
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG253840.14909813951554146TruSeq Adapter, Index 8 (100% over 38bp)
AAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC251810.1479057773062106TruSeq Adapter, Index 8 (100% over 42bp)
CTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC240790.14143295388412871TruSeq Adapter, Index 8 (100% over 44bp)
GCCGGTGTAGCTCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG237460.1394770099643889Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT234730.13787348837253013Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA234120.1375151923391844No Hit
GAAGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA231660.1360702607948721TruSeq Adapter, Index 8 (100% over 40bp)
ATTCCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA217130.12753576675468611TruSeq Adapter, Index 8 (100% over 40bp)
AGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTG207960.12214957884357078No Hit
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC207420.12183239874847783Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT206520.12130376525665626TruSeq Adapter, Index 8 (100% over 48bp)
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT205600.12076338435390531Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT201990.11864297668115437TruSeq Adapter, Index 8 (100% over 43bp)
GGGCTCGTAGATCAGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT199300.11706294991115435Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT197590.11605854627669335TruSeq Adapter, Index 8 (100% over 41bp)
GGGCTCGTAGATCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG197400.11594694587286436Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA195700.11494841594386805Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT195210.11466060437609854TruSeq Adapter, Index 8 (100% over 43bp)
AGGAACTACGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC195130.11461361473238106TruSeq Adapter, Index 5 (100% over 35bp)
GGGCTCGTAGATCAGTGGTAGATCATCCCCAGATCGGAAGAGCACACGTC191540.11250495447055946No Hit
AGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC188550.11074871653661891TruSeq Adapter, Index 5 (100% over 35bp)
AGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT187570.11017309340107986TruSeq Adapter, Index 8 (100% over 43bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT187520.11014372487375644TruSeq Adapter, Index 8 (100% over 48bp)
GCGCTGGTGGTCTAGTGGTAGGACATGAGCAGATCGGAAGAGCACACGTC186690.10965620732018767No Hit
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT183450.10775312674963002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTG183070.10752992594197201No Hit
GGGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT181970.10688381834085678TruSeq Adapter, Index 8 (100% over 41bp)
GGGAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT181160.10640804819821736TruSeq Adapter, Index 8 (100% over 41bp)
GGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC172370.10124506109476003TruSeq Adapter, Index 8 (100% over 42bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA172170.10112758698546634TruSeq Adapter, Index 8 (100% over 40bp)
CGGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA170810.1003287630422693TruSeq Adapter, Index 8 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)