FastQCFastQC Report
Tue 4 Jul 2023
SRR6160955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10325125
Total Bases784.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA7223446.995983099478215TruSeq Adapter, Index 1 (100% over 40bp)
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC7121476.897224004552004TruSeq Adapter, Index 1 (100% over 36bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA2824342.735405140373603TruSeq Adapter, Index 1 (100% over 40bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT1729871.675398602922483TruSeq Adapter, Index 1 (100% over 41bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1643511.591757969031852Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1570851.5213859396375347Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1483221.4365152964249828TruSeq Adapter, Index 1 (100% over 49bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC1138691.1028341061246232TruSeq Adapter, Index 1 (100% over 42bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT915210.8863912059176039Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG901940.8735390612704447TruSeq Adapter, Index 1 (100% over 39bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG858080.8310601566566991TruSeq Adapter, Index 1 (100% over 39bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA844620.8180239948668903Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA715660.6931247805716638TruSeq Adapter, Index 1 (100% over 40bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA602310.583344027312018TruSeq Adapter, Index 1 (100% over 40bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA579980.5617171704943039Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT563330.545591457730536TruSeq Adapter, Index 1 (100% over 43bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC526330.5097565404777181Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG486330.47101608939359085Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT430380.41682788343966776TruSeq Adapter, Index 1 (100% over 41bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC407780.3949395285771358TruSeq Adapter, Index 1 (100% over 36bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT392070.37972421641384485TruSeq Adapter, Index 1 (100% over 43bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC373230.3614774639532209TruSeq Adapter, Index 1 (100% over 42bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA356340.34511930848294814TruSeq Adapter, Index 1 (100% over 40bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT354580.3434147286352465Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC323970.31376859844311816TruSeq Adapter, Index 1 (100% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC320760.31065967724361687TruSeq Adapter, Index 1 (100% over 50bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG318510.3084805268701348Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA305460.2958414547039382No Hit
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA282550.2736528613455043TruSeq Adapter, Index 1 (100% over 40bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT281300.2724422222491253TruSeq Adapter, Index 1 (100% over 41bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC279520.2707182721758817TruSeq Adapter, Index 1 (100% over 42bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA268030.25959007760196606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC255920.24786140603624657TruSeq Adapter, Index 1 (100% over 36bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC254860.24683478408251716TruSeq Adapter, Index 1 (100% over 42bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT249130.24128521446471593No Hit
ATTCCGGTTGATCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA239050.23152262079151584Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA222660.2156487209597947TruSeq Adapter, Index 1 (100% over 40bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT214820.2080555925473057TruSeq Adapter, Index 1 (100% over 48bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT198020.19178460309197226TruSeq Adapter, Index 1 (100% over 48bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC196840.19064175978499048TruSeq Adapter, Index 1 (100% over 36bp)
GCGCTGGTGGTCTAGTGGTAGGACATGAGCAGATCGGAAGAGCACACGTC191110.18509219016718928No Hit
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA190240.1842495853561095TruSeq Adapter, Index 1 (100% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190070.18408493843900195No Hit
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT181390.17567826055374633Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA173260.16780426387089745TruSeq Adapter, Index 1 (100% over 40bp)
GAAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA172430.1670003995109018TruSeq Adapter, Index 1 (100% over 40bp)
GGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC172110.16669047590222877TruSeq Adapter, Index 1 (100% over 42bp)
CGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG170160.1648018789118776TruSeq Adapter, Index 1 (100% over 39bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT166350.16111185094611447Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGACGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC163870.15870994297889857TruSeq Adapter, Index 1 (100% over 38bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC160160.15511676614084574TruSeq Adapter, Index 1 (100% over 42bp)
AAGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC157380.1524243047904989TruSeq Adapter, Index 1 (100% over 38bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC156850.1519109938136342TruSeq Adapter, Index 1 (100% over 36bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT156430.15150421907725087TruSeq Adapter, Index 1 (100% over 43bp)
TTAAGGCGGCCATAGCGGTGGGGTTACTCCCGTAGATCGGAAGAGCACAC156280.1513589423856854No Hit
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC153010.148191910509558Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC152230.1474364717134175TruSeq Adapter, Index 1 (100% over 44bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC147240.14260360044067263No Hit
CAGAACAAAGGGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA145630.14104429728453652Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGGAAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG144310.13976586239876032TruSeq Adapter, Index 1 (100% over 39bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT139450.13505889759203885Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAAGGGCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC139230.13484582511107615TruSeq Adapter, Index 1 (100% over 38bp)
GGGCTCGTAGATCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG138990.13461338240457138Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAAAGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA136580.1322792702267527TruSeq Adapter, Index 1 (100% over 40bp)
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC136110.13182406992651421TruSeq Adapter, Index 1 (100% over 42bp)
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC136050.13176595924988802TruSeq Adapter, Index 1 (100% over 38bp)
GAAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA135790.13151414631784117TruSeq Adapter, Index 1 (100% over 40bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGAGATCGGAAGAGCACACGTCTG130040.1259452064744979Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG127220.12321400467306691TruSeq Adapter, Index 1 (100% over 39bp)
ATTCCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA124270.12035689640561252TruSeq Adapter, Index 1 (100% over 40bp)
GGGAGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT123000.11912688708369149TruSeq Adapter, Index 1 (100% over 41bp)
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT120470.11667655355262042TruSeq Adapter, Index 1 (100% over 41bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA118860.1151172503964843TruSeq Adapter, Index 1 (100% over 47bp)
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT118340.11461362453239064Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT117660.11395503686396048TruSeq Adapter, Index 1 (100% over 43bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC115500.11186305250541762Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC114990.11136911175409499TruSeq Adapter, Index 1 (100% over 42bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA113450.1098776043873561TruSeq Adapter, Index 1 (100% over 47bp)
TTAAGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT110870.10737884529242987TruSeq Adapter, Index 1 (100% over 41bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT110630.1071464025859251TruSeq Adapter, Index 1 (100% over 35bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA110340.10686553431556518Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT106250.10290432319221318TruSeq Adapter, Index 1 (100% over 41bp)
AAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC106250.10290432319221318TruSeq Adapter, Index 1 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)