FastQCFastQC Report
Tue 4 Jul 2023
SRR6160956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17460608
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA9365885.36400565203686TruSeq Adapter, Index 2 (100% over 36bp)
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA7830244.484517377630836TruSeq Adapter, Index 2 (100% over 40bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC4298302.46171267346475TruSeq Adapter, Index 2 (100% over 42bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT3837792.197970425772115Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT2268351.299124291662696TruSeq Adapter, Index 2 (100% over 41bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA2129991.2198830647821657TruSeq Adapter, Index 2 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2059611.1795751900506557TruSeq Adapter, Index 2 (100% over 49bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1896821.086342468715866Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT1746451.0002229017454602TruSeq Adapter, Index 2 (100% over 43bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1403770.8039639856756421Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT993340.5689034425376253Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA969920.5554903930034968TruSeq Adapter, Index 2 (100% over 40bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA840030.48110008540366983TruSeq Adapter, Index 2 (100% over 40bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA826730.47348293942570613Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA740900.4243265755694189TruSeq Adapter, Index 2 (100% over 36bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC692540.3966299455322518Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT680220.3895740629421381TruSeq Adapter, Index 2 (100% over 39bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC675680.3869739243902618Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG658240.37698572695750343Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT629520.36053727338704356TruSeq Adapter, Index 2 (100% over 39bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT597360.34211867078168184TruSeq Adapter, Index 2 (100% over 41bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC594760.34062960465065134TruSeq Adapter, Index 2 (100% over 42bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA564230.3231445319658972No Hit
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA545960.3126809788066945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC520240.2979506784643467TruSeq Adapter, Index 2 (100% over 42bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT519710.2976471380607136TruSeq Adapter, Index 2 (100% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT496710.2844746299785208TruSeq Adapter, Index 2 (100% over 48bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA475040.2720638364941244TruSeq Adapter, Index 2 (100% over 36bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT445210.25497966622926305Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG424320.24301559258417577Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT408890.2341785578142525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT389410.22302201618637796TruSeq Adapter, Index 2 (100% over 43bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT388600.22255811481478766TruSeq Adapter, Index 2 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC369130.21140730036434013TruSeq Adapter, Index 2 (100% over 50bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA359790.20605811664748444TruSeq Adapter, Index 2 (100% over 36bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG357530.2047637745489733TruSeq Adapter, Index 2 (100% over 35bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA347570.19905950583164114TruSeq Adapter, Index 2 (100% over 36bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA328150.1879373272683288TruSeq Adapter, Index 2 (100% over 36bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC326210.18682625484748297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG317710.1819581540344987TruSeq Adapter, Index 2 (100% over 38bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA304600.1744498244276488TruSeq Adapter, Index 2 (100% over 40bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC291870.16715912756302642Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT291640.16702740248220452Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT291290.1668269512722581TruSeq Adapter, Index 2 (100% over 41bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT288610.1652920677218113No Hit
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT288510.16523479594754087TruSeq Adapter, Index 2 (100% over 43bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT284240.16278929118619465Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA278590.15955343593991686TruSeq Adapter, Index 2 (100% over 47bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC278580.15954770876248983TruSeq Adapter, Index 2 (100% over 42bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA270170.1547311525463489TruSeq Adapter, Index 2 (100% over 47bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC267760.15335090278643218TruSeq Adapter, Index 2 (100% over 44bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT264630.15155829625176856TruSeq Adapter, Index 2 (100% over 43bp)
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA262880.1505560402020365TruSeq Adapter, Index 2 (100% over 40bp)
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC262470.15032122592752784Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGGTGTAGCTCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG258750.14819071592466884Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCTCGTAGATCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG257160.14728009471376943Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC254650.1458425731795823TruSeq Adapter, Index 2 (100% over 42bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC252370.1445367767262171TruSeq Adapter, Index 2 (100% over 42bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT245260.14046475357559143TruSeq Adapter, Index 2 (100% over 41bp)
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT232390.1330938762269905Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA228720.1309920021112667TruSeq Adapter, Index 2 (100% over 40bp)
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG226940.1299725645292535TruSeq Adapter, Index 2 (100% over 38bp)
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA225190.12897030847952143TruSeq Adapter, Index 2 (100% over 40bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC224590.12862667783389903TruSeq Adapter, Index 2 (100% over 42bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220930.12653053089560226No Hit
ATTCCGGTTGATCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC212510.1217082475020343Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT211850.12133025379184963TruSeq Adapter, Index 2 (100% over 48bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGAGATCGGAAGAGCACACGTCTG207280.1187129337076922Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA203290.11642778991430308TruSeq Adapter, Index 2 (100% over 40bp)
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT197340.1130201193452141TruSeq Adapter, Index 2 (100% over 41bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC190840.10929745401763787No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT187790.10755066490239057TruSeq Adapter, Index 2 (100% over 48bp)
GGGCTCGTAGATCAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT186740.10694931127255133Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC180370.10330109925152664TruSeq Adapter, Index 2 (100% over 42bp)
GGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT176090.10084986731275337TruSeq Adapter, Index 2 (100% over 43bp)
GGGAGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT174850.10013969731180036TruSeq Adapter, Index 2 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)