FastQCFastQC Report
Tue 4 Jul 2023
SRR6160958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20619543
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA19142359.283595664559588TruSeq Adapter, Index 4 (100% over 40bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC5402332.62000472076418TruSeq Adapter, Index 4 (100% over 42bp)
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA4857752.3558960545342833TruSeq Adapter, Index 4 (100% over 36bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT4074011.9758003366029984Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA4010561.9450285585863858TruSeq Adapter, Index 4 (100% over 40bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3153381.5293161443975747Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA1899050.9209951937344101TruSeq Adapter, Index 4 (100% over 40bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA1694190.8216428463036256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT1670070.8099452058661049TruSeq Adapter, Index 4 (100% over 41bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG1645560.798058424476236Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA1588770.7705165919535656No Hit
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1560680.7568935936165025Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT1500250.7275864455385844TruSeq Adapter, Index 4 (100% over 43bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA1459090.7076248004138598TruSeq Adapter, Index 4 (100% over 40bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1440430.6985751333092106Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT1238800.600789260945308TruSeq Adapter, Index 4 (100% over 41bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA1206180.5849693177002031TruSeq Adapter, Index 4 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1125730.5459529340684224TruSeq Adapter, Index 4 (100% over 49bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA1034820.5018636930993087TruSeq Adapter, Index 4 (100% over 39bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC986280.47832291918399933TruSeq Adapter, Index 4 (100% over 42bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT930500.4512709132302302Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG916350.444408491497605Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT823480.39936869599874253Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT690500.33487648101609235No Hit
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT631630.30632589674756616Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAACTACGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG611600.29661181142569454TruSeq Adapter, Index 4 (100% over 35bp)
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT601870.29189298715301304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC582170.2823389441754359Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA572070.2774406784864243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA562920.27300314075826027TruSeq Adapter, Index 4 (100% over 40bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA532600.25829864415520754TruSeq Adapter, Index 4 (100% over 36bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA528340.2562326429834066TruSeq Adapter, Index 4 (100% over 36bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC518260.25134407683041277TruSeq Adapter, Index 4 (100% over 42bp)
AGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTG496630.24085402862711358No Hit
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG496160.24062608953069425No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC493980.23956884010474916TruSeq Adapter, Index 4 (100% over 50bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA433040.21001435385837602TruSeq Adapter, Index 4 (100% over 39bp)
GTACGAAAGTGCGATAAGGGGACTCCGAGTAGATCGGAAGAGCACACGTC419220.20331197447004523No Hit
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA404820.19632830853719696TruSeq Adapter, Index 4 (100% over 40bp)
AGGAACTACGAACGGGTGCCACTGGTGTATCGGTTGTCCGAGAGGGCATG383410.18594495523009408No Hit
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC376900.1827877562562856No Hit
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTAGATCGGAAGAGCA354710.17202612104448678No Hit
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT354110.17173513496395143Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA353480.1714295995793893Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG350770.17011531244897135No Hit
ATTCCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA347780.16866523181430354TruSeq Adapter, Index 4 (100% over 40bp)
GCGCTGGTGGTCTAGTGGTAGGACATGAGCAGATCGGAAGAGCACACGTC340470.16512005139978125No Hit
TCCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT303600.14723895675088436TruSeq Adapter, Index 4 (100% over 41bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC295470.14329609535963042TruSeq Adapter, Index 4 (100% over 42bp)
AGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG292270.14174416959677527TruSeq Adapter, Index 4 (100% over 35bp)
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC290360.14081786390707107Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA289830.1405608262025982No Hit
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT288770.14004675079365242TruSeq Adapter, Index 4 (100% over 43bp)
AGGAACTACGAACGGGTGCCACTGGTGTAAGATCGGAAGAGCACACGTCT284880.1381601910381816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA283810.13764126586122688TruSeq Adapter, Index 4 (100% over 40bp)
CGGGAAACTGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG279630.13561406283349733TruSeq Adapter, Index 4 (100% over 35bp)
CGAAAGTGCGATAAGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGA275800.13375660168608003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC274720.13323282674111642TruSeq Adapter, Index 4 (100% over 44bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT264940.1284897536283903TruSeq Adapter, Index 4 (100% over 48bp)
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC264730.12838790850020293TruSeq Adapter, Index 4 (100% over 38bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT263570.12782533541116795TruSeq Adapter, Index 4 (100% over 41bp)
ATAAGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA263140.12761679538678428Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC259930.1260600198559202TruSeq Adapter, Index 4 (100% over 42bp)
CTGTACGAAAGTGCGATAAGGGGACTCCGAGTAGATCGGAAGAGCACACG258640.1254343997827692No Hit
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT257170.1247214838854576Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTAAGGCGGCCATAGCGGTGGGGTTACTCCCGTAGATCGGAAGAGCACAC255140.12373698097964635No Hit
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA255040.12368848329955713TruSeq Adapter, Index 4 (100% over 36bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT241960.11734498674388662TruSeq Adapter, Index 4 (100% over 48bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC240690.11672906620675348TruSeq Adapter, Index 4 (100% over 42bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTG238550.11569121585284407No Hit
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT232890.11294624715979398TruSeq Adapter, Index 4 (100% over 43bp)
GCAGTGACTCGGAGGCTCCGACTAGATCGGAAGAGCACACGTCTGAACTC232760.112883200175678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA229370.1112391288206533TruSeq Adapter, Index 4 (100% over 40bp)
GCGGAAAGCTCAGTAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTC229280.111195480908573Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCCGGTTGATCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT228870.11099664042020718Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT228360.11074930225175214TruSeq Adapter, Index 4 (100% over 41bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA227440.11030312359493127TruSeq Adapter, Index 4 (100% over 36bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC222510.10791218796653253TruSeq Adapter, Index 4 (100% over 42bp)
GAAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA222480.10789763866250576TruSeq Adapter, Index 4 (100% over 40bp)
CGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA220160.10677249248443577TruSeq Adapter, Index 4 (100% over 39bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG219440.10642330918779336TruSeq Adapter, Index 4 (100% over 35bp)
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT217430.10544850581799994Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCCGGTAGTCTAGTGGTAGGACCTGAGCAGATCGGAAGAGCACACGTC215050.10429426103187642No Hit
AAGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC211460.10255319431667327TruSeq Adapter, Index 4 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)