FastQCFastQC Report
Tue 4 Jul 2023
SRR6160959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39663230
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA21976175.540690962385061TruSeq Adapter, Index 5 (100% over 36bp)
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA20116605.071851183073088TruSeq Adapter, Index 5 (100% over 40bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT6357311.602822059625502Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT6331531.596322336834393TruSeq Adapter, Index 5 (100% over 41bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA5604861.4131123461200714TruSeq Adapter, Index 5 (100% over 40bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5180651.3061593823801037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC5066791.277452693590512TruSeq Adapter, Index 5 (100% over 42bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT3795820.9570123260258936Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3544860.8937396172727233TruSeq Adapter, Index 5 (100% over 49bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2611390.6583906555265419Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT2599140.6553021526486875TruSeq Adapter, Index 5 (100% over 43bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA2585370.6518304232912953Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA2562290.6460114317467337TruSeq Adapter, Index 5 (100% over 40bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG2158020.5440857943238612Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA2027420.5111585718056749TruSeq Adapter, Index 5 (100% over 40bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA1967660.49609172021542375No Hit
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG1767470.4456192801241855TruSeq Adapter, Index 5 (100% over 39bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1676840.4227694012817413Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA1676730.42274166778651157TruSeq Adapter, Index 5 (100% over 36bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG1573690.39676294643678794TruSeq Adapter, Index 5 (100% over 39bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1549830.3907472991987794Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT1472400.3712254397839006TruSeq Adapter, Index 5 (100% over 41bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG1430690.3607094026381613Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA1178400.2971013707153956TruSeq Adapter, Index 5 (100% over 36bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC1164150.29350862246972825TruSeq Adapter, Index 5 (100% over 42bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT1065550.2686493258365494TruSeq Adapter, Index 5 (100% over 43bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG975670.245988539007035TruSeq Adapter, Index 5 (100% over 39bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT948060.2390274317043771TruSeq Adapter, Index 5 (100% over 48bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA845090.21306635894252687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT800620.20185446318920572TruSeq Adapter, Index 5 (100% over 48bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT790380.19927272690600334Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC768570.19377393117000305No Hit
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT754480.19022152255376076Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC730960.18429159702828035TruSeq Adapter, Index 5 (100% over 50bp)
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA728150.18358313228650314TruSeq Adapter, Index 5 (100% over 40bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC718500.18115014838680563TruSeq Adapter, Index 5 (100% over 42bp)
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA701130.17677077736735006TruSeq Adapter, Index 5 (100% over 40bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT700310.17656403676654675Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC694320.17505382188994695TruSeq Adapter, Index 5 (100% over 35bp)
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA687800.1734099819908767TruSeq Adapter, Index 5 (100% over 36bp)
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT671520.1693054246968792TruSeq Adapter, Index 5 (100% over 41bp)
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC669130.1687028514823427Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC661070.1666707426500565TruSeq Adapter, Index 5 (100% over 42bp)
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT661050.16666570019637836TruSeq Adapter, Index 5 (100% over 38bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT652600.1645352635173686TruSeq Adapter, Index 5 (100% over 43bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG642360.16195352723416626No Hit
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC636010.1603525481913601TruSeq Adapter, Index 5 (100% over 42bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA624120.15735480947971206Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA620290.15638917960035026TruSeq Adapter, Index 5 (100% over 40bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA618560.15595300735719203TruSeq Adapter, Index 5 (100% over 36bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC582660.14690180300494943TruSeq Adapter, Index 5 (100% over 42bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC569240.14351831658692446TruSeq Adapter, Index 5 (100% over 44bp)
GGGCTCGTAGATCAGCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG561090.14146351671308666Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT551080.13893976864718277TruSeq Adapter, Index 5 (100% over 41bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA550320.13874815540741386TruSeq Adapter, Index 5 (100% over 36bp)
ATTCCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA544610.13730853488230788TruSeq Adapter, Index 5 (100% over 40bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA532250.1341922985092238No Hit
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC526410.13271990203520995Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGGTGTAGCTCAGCGGTAGAGCAGATCGGAAGAGCACACGTCTGAACT523780.1320568193765359Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA514670.12975998172614786TruSeq Adapter, Index 5 (100% over 47bp)
AGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC503700.1269941958836938TruSeq Adapter, Index 5 (100% over 35bp)
AGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTG494210.12460155161342129No Hit
ATTCCGGTTGATCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA493790.12449566008618058Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT485880.12250136965648033TruSeq Adapter, Index 5 (100% over 41bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT484450.12214083421849406No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA483120.12180551104889843TruSeq Adapter, Index 5 (100% over 47bp)
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT477540.12039866647270028TruSeq Adapter, Index 5 (100% over 38bp)
GGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC466970.1177337297038088TruSeq Adapter, Index 5 (100% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG464980.1172320055628349TruSeq Adapter, Index 5 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG460570.11612014452680733No Hit
GGGCTCGTAGATCAGTGGTAGATCAGATCGGAAGAGCACACGTCTGAACT460190.11602433790692285Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT454610.1146174933307247TruSeq Adapter, Index 5 (100% over 41bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA440790.11113315783913716TruSeq Adapter, Index 5 (100% over 40bp)
ATTCCGGTTGATCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC435820.10988010810012196Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC435660.10983976847069692TruSeq Adapter, Index 5 (100% over 42bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT433300.10924475893667762TruSeq Adapter, Index 5 (100% over 43bp)
GGGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT430900.10863966449530207TruSeq Adapter, Index 5 (100% over 41bp)
GGGAGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT425330.10723534114594298TruSeq Adapter, Index 5 (100% over 41bp)
GAAGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA420180.1059369093238246TruSeq Adapter, Index 5 (100% over 40bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA413950.10436618500308725Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCTCGTAGATCAGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT405030.10211725066264145Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)