FastQCFastQC Report
Tue 4 Jul 2023
SRR6160960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6160960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815990
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC13571426.848721663666564TruSeq Adapter, Index 6 (100% over 36bp)
ATTCCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA12043966.0778997163401876TruSeq Adapter, Index 6 (100% over 40bp)
GGGCTCGTAGATCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT4577972.310240366491909Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA3740241.8874858132245727TruSeq Adapter, Index 6 (100% over 40bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC3002151.5150138852512542TruSeq Adapter, Index 6 (100% over 42bp)
GGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT2387271.204719017318842TruSeq Adapter, Index 6 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2379901.2009997986474559TruSeq Adapter, Index 6 (100% over 49bp)
ATTCCGGTTGATCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2150311.0851388197107488Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCTCGTAGATCAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1925640.9717606841747498Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCA1423350.7182835679670811Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1421060.717127935571223Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAG1318540.6653919385304494Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT1296550.654294839672406TruSeq Adapter, Index 6 (100% over 43bp)
AGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT1148730.5796985161982823TruSeq Adapter, Index 6 (100% over 39bp)
GGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT1031600.5205896854005275TruSeq Adapter, Index 6 (100% over 39bp)
TAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTGTGA1029640.5196005851839852No Hit
ATTCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA1015590.5125103514888734TruSeq Adapter, Index 6 (100% over 40bp)
ATTCCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA968460.4887265284247721TruSeq Adapter, Index 6 (100% over 40bp)
GCCGCCTTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC943390.47607512922644796TruSeq Adapter, Index 6 (100% over 36bp)
ATTCCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT854600.43126788013114664TruSeq Adapter, Index 6 (100% over 41bp)
GGGCTCGTAGATCAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT788400.3978605156744629Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGAATCTGAGACAAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG744340.37562594652096615Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC693760.3501011052185634TruSeq Adapter, Index 6 (100% over 50bp)
ATTCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC643380.32467719250968535TruSeq Adapter, Index 6 (100% over 42bp)
GCCGCCATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC627160.3164918835748302TruSeq Adapter, Index 6 (100% over 36bp)
ATTCCGGTTGATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG622590.3141856652127903Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCCTTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC605630.30562692048189366TruSeq Adapter, Index 6 (100% over 36bp)
GCCGCCTTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC504920.2548043272125188TruSeq Adapter, Index 6 (100% over 36bp)
ACGGGTTGTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG480360.24241029592768265Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGAATCTGAGACAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT442410.22325909530636623Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTAAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA439890.22198739502795473TruSeq Adapter, Index 6 (100% over 40bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTA436330.2201908660632146No Hit
ACGGGTTGTGAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC427520.2157449615184505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT421850.21288363589202458TruSeq Adapter, Index 6 (100% over 39bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC410190.20699949888953315TruSeq Adapter, Index 6 (100% over 42bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA399480.20159477270628415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCTCGTAGATCAGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT391160.19739614321565563Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT373960.18871628417252936TruSeq Adapter, Index 6 (100% over 48bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC371120.18728309814447827TruSeq Adapter, Index 6 (100% over 44bp)
GAAAGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA355240.17926936781861516TruSeq Adapter, Index 6 (100% over 40bp)
GAAGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA353400.17834082475818772TruSeq Adapter, Index 6 (100% over 40bp)
CGTAGAAGACGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC348480.1758579813574795TruSeq Adapter, Index 6 (100% over 35bp)
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTAGATCGGAAGAGCA347950.17559052058463898No Hit
ACGGGTTGTGAGAGCAAGAGCCCGGAGACGGAATCTGAGACAAGATTCCG340960.17206306624094986No Hit
GAGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT329080.16606790778558125TruSeq Adapter, Index 6 (100% over 41bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT327000.1650182504129241TruSeq Adapter, Index 6 (100% over 43bp)
GGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA322260.16262624274638815TruSeq Adapter, Index 6 (100% over 40bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT319750.16135958889765284TruSeq Adapter, Index 6 (100% over 43bp)
TAGACGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA309650.15626269492465428TruSeq Adapter, Index 6 (100% over 38bp)
GGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT307490.15517266611458727TruSeq Adapter, Index 6 (100% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302980.15289672633060472No Hit
GTACGAAAGTGCGATAAGGGGACTCCGAGTAGATCGGAAGAGCACACGTC302350.15257880126100185No Hit
GAAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT301180.1519883689888822TruSeq Adapter, Index 6 (100% over 41bp)
AGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC295130.1489352790347593TruSeq Adapter, Index 6 (100% over 35bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT292350.14753237158476562TruSeq Adapter, Index 6 (100% over 48bp)
TTAAGGCGGCCATAGCGGTGGGGTTACTCCCGTAGATCGGAAGAGCACAC289000.1458418176432265No Hit
ATTCCGGTTGATCCTGCCGGAGGCCATTGCTATCGGAGTCCGATTTAGCC285170.14390903507722805No Hit
ACGGGTTGTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC284960.14380306005402707TruSeq Adapter, Index 6 (100% over 36bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC280050.1413252630829951TruSeq Adapter, Index 6 (100% over 42bp)
CGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC277980.1402806521400142TruSeq Adapter, Index 6 (100% over 42bp)
CGATAGACTTGGGGTGTACGCGTCGAGGCAGATCGGAAGAGCACACGTCT275610.13908464830674622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGAAAGCTCAGTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA275570.13906446258804128Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC269050.13577419043913524TruSeq Adapter, Index 6 (100% over 42bp)
TAAACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGC267640.13506264385478597No Hit
AGGTAGACGGTGGGGTAACGGCCCACCGTGCCCATAATCGGTACGGGTTG264620.13353862209256262No Hit
AACTCGATCGTAGTTCGGATTGAGGGCTGAAACTCGCCCTCATGAAGCTG262550.13249401114958173No Hit
AGGGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA260990.13170676812008889TruSeq Adapter, Index 6 (100% over 40bp)
GGGCTCGTAGATCAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT257980.13018779278754178Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA257140.12976389269473793TruSeq Adapter, Index 6 (100% over 47bp)
CTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC242870.12256263754674886TruSeq Adapter, Index 6 (100% over 44bp)
GAAAGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA236450.11932282969460521TruSeq Adapter, Index 6 (100% over 38bp)
ATAAGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA233450.11780890079173434Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGAAAGTGCGATAAGGGGACTCCGAGTAGATCGGAAGAGCACACGTCTGA221050.11155132799320144Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCAGTGACTCGGAGGCTCCGACTAGATCGGAAGAGCACACGTCTGAACTC220420.11123340292359858Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT219300.11066820279986012TruSeq Adapter, Index 6 (100% over 43bp)
GCCGGTGTAGCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC217340.10967910258331783Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGAAACTGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC217330.10967405615364158TruSeq Adapter, Index 6 (100% over 35bp)
CCGCCCTTAGCTCAGACTGGTAGAGCAGTCGAGATCGGAAGAGCACACGT215860.10893223099123486No Hit
AAGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC215760.10888176669447248TruSeq Adapter, Index 6 (100% over 42bp)
CAGAACAAAGGGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG215550.10877579167127152Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGATAGACTTGGGGTGTACGCGTCGAGGCAACAGATCGGAAGAGCACACG212770.10737288422127786No Hit
ATTCCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA209660.1058034445919684TruSeq Adapter, Index 6 (100% over 40bp)
AAGAAAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA203610.1027503546378455TruSeq Adapter, Index 6 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)