FastQCFastQC Report
Thu 28 Sept 2023
SRR6181538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10007854
Total Bases750.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2898842.8965650378192964No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC2305362.303550791208585No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2129952.128278450105287No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1515531.514340636863807No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1277101.2760977528249313No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG991450.9906719262691082TruSeq Adapter, Index 6 (100% over 49bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC837090.8364330654703797No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA832650.8319965499097008No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA809310.8086748667596468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG783040.7824254830256316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG776690.7760804664016882No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA675030.6745002475056091Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC518000.517593482079175No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA513460.5130570449968594No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA503300.5029050183985498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC474470.4740976437106297No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA426950.42661493662877176No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC415690.4153637732924561No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT399620.39930638476540525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC364400.36411402484488686No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAG357770.3574892279603599No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA329520.32926139809793387Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA300980.3007437958227608No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC296880.29664701343564764No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG296600.2963672331750643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG296040.2958076726538976No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT292430.2922005057228053No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT283810.28358727055770394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA278750.2785312415628765No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG273720.27350518902454013No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT269920.2697081712023377No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGC249170.24897445546267963No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT244460.2442681517935813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG230590.23040903674254243No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG220300.22012711216610475No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA217520.21734929386459872No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT206050.20588829533284558No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT204210.2040497393347265No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG196660.19650566445114007No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG196600.19644571153815796No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC185730.1855842421362262No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC185710.1855642578318988No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT184440.18429525450711012No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA173030.1728942088883391No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC166360.16622944339515744No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG164520.16439088739703836No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT164250.16412109928861873No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC158670.15854547838127933No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTAGATCGGAAGAG157380.15725649075216325No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT150350.15023200778108875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA149480.14936269054284765No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT146270.14615520969830295No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA146160.14604529602450236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCAGATCGGAAGAGCA141930.14181861565926124No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA134990.13488406205765993No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC133930.1338248939283087No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC132630.13252591414702894No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC129330.12922850393301102No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGC122920.12282353439608532No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACAC113790.11370069947063577No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC113090.11300124881917742Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG110570.11048322647392737No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGA109850.10976379151814165No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGAGATCGGAAGAGC108290.10820501578060593No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA107420.10733569854236483No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACAGATCGGAAGAGCACA104250.1041681863064749No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG100520.10044111354941829No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)