FastQCFastQC Report
Thu 28 Sept 2023
SRR6181540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22618870
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC8213163.6311097769251957No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT6979603.0857421259329048No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4370151.9320814877135772No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3967871.7542299858480996No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3528211.5598524594729977No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3123571.380957580993215No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2782691.2302515554490565No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1945320.8600429641268552TruSeq Adapter, Index 12 (100% over 49bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1817530.8035458888971907No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1766960.7811884501745666No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1635820.7232103106830713No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1331980.5888799926786793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1240450.5484137801755791No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1185830.5242658010767116No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1132800.5008207748662953No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1092290.4829109500165128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1022660.452126918807173Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC926070.4094236361056056No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA861270.38077499008571164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA820700.36283863871183664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT773500.34197110642574097No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA715740.3164349058993663No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG703420.3109881262857075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC675400.2986002395345126Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA626390.2769324904382933No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG624520.27610574710407726No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG603540.2668303058464017No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA596510.26372228144023113Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAG579790.2563302233931226No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC568440.25131228925229243No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT496150.21935224880818538No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT480940.21262777495073804No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC467850.20684057161122552No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG463970.20512518971991084No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGC460100.20341422891594496No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG432510.19121644892074627No Hit
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC429270.18978401661975156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA386980.1710872382218917No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT385820.17057439208943684No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG374800.16570235383111537No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT365520.1615995847714762No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG363320.1606269455547514No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT351760.15551616857959746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC346480.153181834459458No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC343680.15194393000180823No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC334900.14806221530960653No Hit
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG331410.1465192558248931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCAGATCGGAAGAGCA331170.14641314972852312No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC317370.1403120491872494No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG316530.1399406778499545Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG308240.1362755964378415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG304400.13457789889592184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG298510.13197387844750863Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT291300.12878627446906057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT267570.11829503419047901No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA254040.1123133030076215No Hit
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG249730.11040781436031068Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT249290.11021328651696571No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA241430.10673831186084892No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA240000.10610609636997781No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG233930.10342249634928713No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC231400.1023039612500536No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACAC230590.10194585317480492No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTAGATCGGAAGAG228590.10106163570505512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)