FastQCFastQC Report
Thu 28 Sept 2023
SRR6181541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26156283
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC9507313.634809273167751No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT8047333.076633633303325No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5228471.9989346345579762No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4623921.7678046991615743No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4166141.5927874767221324No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3692551.4117258174642018No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3256791.2451272223962404No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2138030.8174058982310292No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC2078870.7947880056199117No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1962000.7501065805106941No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1902740.7274504561676444TruSeq Adapter, Index 12 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1523890.582609539742325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1451690.5550062292872424No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1400140.5352977714761689No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1372390.5246884658649702No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1264580.48347083566881427Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1215400.46466846990453503Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1101440.4210995881945458No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA999460.38211086797003996Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA949580.36304088008223495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT919340.3514796043459233No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA878110.3357166612702577No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG821120.3139283972420699Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC773980.2959059588092085Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA739160.2825936697503999No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG732540.28006272909648516No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAG718780.2748020427826079No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG699750.2675265441959012No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA685340.2620173516244644Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC658130.25161449736569985No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT578780.22127761807746155No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGC568690.21742003632549775No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT568490.21734357286163328No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG563930.2156002058855228No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC543660.20785063382285626No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG517040.197673346782492No Hit
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC481840.1842157771423409Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA472580.1806755187654148No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT462940.17698997980714615No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG440330.168345785217265No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG433700.165811021390157No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT429110.16405618489446686No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT414180.15834818731698233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC403160.15413505045804865No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC403100.1541121114188893No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCAGATCGGAAGAGCA400130.15297662898050154No Hit
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG393470.15043039563381386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC392190.1499410294650811No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG374250.14308225675643593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC369570.14129301170200675No Hit
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG362880.1387353088357394Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG362870.13873148566254617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG356160.13616613644989237Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT340410.13014463867056342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT321570.12294178037452798No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA313420.11982589422204983No Hit
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG294580.11262303592601441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT292890.11197691965635942No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA288690.11037118691520503No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA287450.10989711343924516No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG284270.10868134436379971No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACAC278900.10662830035903803No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTAGATCGGAAGAG273320.10449496971721861No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC272700.10425793297923869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)