FastQCFastQC Report
Thu 28 Sept 2023
SRR6181542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21199342
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC4056931.9137056235047294Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG3258911.5372694114751297TruSeq Adapter, Index 8 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2352561.1097325567935081Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2295391.0827647386414163Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2001660.944208551378623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1833360.8648192948630198No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1800620.8493754192936744No Hit
ACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT1752880.8268558524127776Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1649300.7779958453427469Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1420510.6700726843314287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1181740.5574418300341586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1138940.5372525241585329Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1074870.5070298880031276Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG955980.4509479586677737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG930380.43887211216272654Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG873670.41212128187752245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG857360.4044276468580959Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA798360.3765965943659949No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA796610.375771097046314No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG733760.34612395045091493Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA660810.3117125050390715Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG636960.3004621558537053Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGA549260.25909294731883664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG546210.2576542234188212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC489940.23111094674542254Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT478480.22570511858339753No Hit
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT471930.22261540004402022Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC471500.22241256355975575No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG466540.2200728682994029Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC464420.21907283726070367Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG411040.19389281044666387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT391810.18482177418525536No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA384710.1814726136311212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG373680.17626962195336063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT356600.16821276811327446Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG345190.16283052558895458No Hit
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA342040.16134463041352887Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG340980.16084461489417926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG329000.15519349610002045No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA324440.15304248594130893No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG304900.14382521872612838No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA302400.14264593684086987No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA299820.1414289179352831Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA288270.13598063562538876No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC278390.13132011361484708Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC278220.13123992244664953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT268630.12671619713479784Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC267270.12607466778921725No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG265370.12517841355642076No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC258370.1218764242776969Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG256760.12111696674359043Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC251320.11855084936126792Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA243400.11481488434876894Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC235740.11120156465233685Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC231590.10924395672280772Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG228940.10799391792443369No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG224510.1059042304237556No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA223460.10540893203194703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC221930.10468721151816882Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA221720.1045881518398071No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC221120.10430512418734507No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC221000.10424851865685265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)