FastQCFastQC Report
Thu 28 Sept 2023
SRR6181543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20204752
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC3804231.8828392449459415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2920411.4454074962167316TruSeq Adapter, Index 8 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2238961.1081353535049576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2157161.0676498281196423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1877140.9290586689705471Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1700080.8414258190350469No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1697940.8403666622584627No Hit
ACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT1637260.8103341233785002Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1573240.7786485080341495Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1309520.6481247579777272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1144450.5664261555895366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1055440.5223721627466648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1021120.5053860596754665Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG910360.45056727249114464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG854720.42302919629995955Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG847670.41953991813411023Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG802510.3971887405497479Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA763650.3779556413263573No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA705280.34906639784541776No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG651220.3223103159098414Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA627260.3104517194766855Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG609570.30169635341230616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGA509640.25223769141041674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG509620.2522277927489533Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC445600.22054217740460264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT443030.21927019940655543No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG440880.21820609329923968Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC432050.21383583426314762No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC421550.2086390369948614Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT419920.20783229608559414Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG393170.19459283637829358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT362840.17958151626904403No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA358510.17743845606221745Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG355740.17606749144953623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT328560.16261521052077255Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA327370.16202624016370012Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG323160.15994257192565392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG317880.1573293252993157No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA303900.15041016093639753No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG290790.14392158834713736No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA278770.13797249280763257No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA278480.13782896221641325Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG272540.13488905976178278No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA271590.13441887334227118No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC267730.1325084316798345Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC260080.12872219367008317Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT252300.12487161436081967Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG245630.12157041076277503No Hit
AAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG243630.12058054461643478Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC239400.1184869777169252Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC239290.1184325350788765No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC235480.11654684007009837Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG221010.10938515850132681No Hit
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC219190.1084843803081572Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG213800.1058166910437703No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA211940.10489611552767389Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA209900.10388645205840685No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA209300.1035894922145048Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC208020.10295597788084704Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC207300.10259962606816454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)