FastQCFastQC Report
Thu 28 Sept 2023
SRR6181544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21265012
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC4121791.9382965784359774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG3257291.531760245420976TruSeq Adapter, Index 8 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2316691.089437428956071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2284681.074384533617945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2050440.9642317624838397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1875040.8817488558200673No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1791510.842468370109549No Hit
ACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT1756850.8261692963070042Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1642400.7723484943248563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1431780.673303170484926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1195600.5622381026636618Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1148250.5399714799126377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1082690.5091414949589494Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG972470.4573098759596279Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG955000.4490945032149523Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG881640.4145965212716551Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG861650.40519610334572115Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA820400.38579804234298104No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA808720.3803054519790537No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG739590.3477966530185828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA668050.31415453703952767Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG633220.2977755197128504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGA553660.2603619504188382Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG540840.25433326818719876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC498400.23437560251553116Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT488770.22984703700143694No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC484340.2277638028137487No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC475970.2238277598902836Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG468310.22022559874407782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT464180.2182834413636823Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG412590.19402293306958868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT407250.19151176589977942No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA376680.17713603923665786Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG375820.17673161905575224Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT365150.1717139872763768Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA355080.16697850911158668Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG346550.16296722522423218No Hit
CAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG339170.15949673576483286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA334830.15745582461933247No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG333280.15672692778165373No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG315500.14836577566944237No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA309750.14566180352966646No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA303900.14291080578745968Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA300690.1414012839494283No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC285240.13413582837385657Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC279530.131450666475053Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC273140.12844573047971947No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT271960.12789082837103502Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG269760.12685626511755554No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC264470.12436861074896172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG257400.12104390065709814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC256690.12071001887983886Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC252180.11858916421020596No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA236560.11124376511050171Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC236420.11117792926709846Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC231310.10877492098288023Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG228260.10734064010873823No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA226560.1065412048674132No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG225830.10619791796966772No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC225590.10608505652383361No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC222860.10480125757747044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC221350.10409117098076409Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA220480.10368204823961538Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA215150.10117558363004919Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)