FastQCFastQC Report
Thu 28 Sept 2023
SRR6181545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21572733
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC4166291.931275930592568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG3323841.5407598100806235TruSeq Adapter, Index 8 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2461641.1410886140388425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2390851.1082740420511392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2077840.9631788424767507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1903130.8821923490176233No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1884020.8733339442897661No Hit
ACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT1764720.8180326526082717Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1729290.801609142429937Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1419220.6578767743521416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1242770.5760837071501326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1167430.5411599911796062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1113630.516221101888203Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG999600.4633627088417587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG957740.4439585841997859Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG916210.4247074304400838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG871850.40414443547787854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA821340.38073061952790127No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA813060.37689244102729125No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG768080.3560420462256683Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA689030.31939856669991695Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG662250.3069847478295865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG565750.2622523534686124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGA559830.2595081485503019Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC503630.23345674375147554Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT499040.23132905784352867Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT486090.22532610958472438No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG485160.22489500982559787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC467760.21682927239677977Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC459700.2130930744843502No Hit
TAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG427080.1979721345459567Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT408040.18914617818706606No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA397490.18425574543568496Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG389750.18066788292424515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT367700.1704466466997946Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA359240.16652502953612786Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG351040.16272393488576528No Hit
CAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG344350.15962279790882314Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA336780.15611373857915917No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG332470.15411584614707835No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA314390.145734895991157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA313990.1455494767399198No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG312920.14505348024286027No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA304990.14137754358708282No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC300090.13910615775942714Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC289460.1341786411577986Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT282200.13081328174784343Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG273700.12687312265905298No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC272070.12611753921026142No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC266540.12355411806190712Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC264760.1227290023939016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG264510.12261311536187836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA246740.11437586512566582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC238660.11063039625067442Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG236940.10983309347035446No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA236740.10974038384473585No Hit
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC235630.10922584542255263Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA232700.10786764940724014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC230900.10703326277667274No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG229920.10657898561114162No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC228220.10579095379338352Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC226920.10518834122686263No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA219810.10189251403612143Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC219480.10173954315385074Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)