FastQCFastQC Report
Thu 28 Sept 2023
SRR6181546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16630092
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC3343362.0104278436944307Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2389461.4368290927073644TruSeq Adapter, Index 8 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2241711.3479841242008763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2236031.3445686289648908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1756361.0561336642034211No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1586720.9541258100075454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1554190.9345648839465229Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1511200.9087141550389499No Hit
ACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT1386080.8334770487138616Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1078500.6485231711285783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT946310.5690347353460221Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG936170.5629373547662875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG891420.536028303391226Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG870780.5236170671815887Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG745030.44800112951870624Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG712530.4284582430451978Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA693060.4167505507486068No Hit
TCGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG674380.405517900923218Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG661870.3979953929298768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA610250.36695527601410743No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA523190.314604393048457Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG483820.2909304410342408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG482180.2899442769168084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGA424120.25503166188136545Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC409810.24642677863718374Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT403110.242397937425722Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC402540.24205518526295586No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT400440.24079241413697533No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC368100.221345738796875Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT357650.21506194914616225Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT353320.21245823534830716No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG349580.2102093001048942Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA342450.20592189147239834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG304530.18311985285469257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA287390.17281323518835615No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG283870.17069659025337924No Hit
CTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG276650.16635506285834137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA274670.16516445008241687Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG271420.163210161435066No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA264480.15903700352349223No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG262180.15765396848075164No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC259150.15583197014183686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA250440.1505944765669366Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA249980.1503178695583885No Hit
CAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG246740.14836959410687564Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA242300.1456997351548025Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC239060.14375145970328967No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC234300.14088917848440044No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG230430.13856207169509346No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC228530.13741956448587295Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC227910.13704674634391678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC224920.13524880078835402Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT220070.1323324008069228Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA215570.1296264626798216Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC205090.1233246334415949Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA203350.12227833736578246Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC199360.11987907222641943Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA198720.11949422769278727Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC192780.11592238936501374Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC188670.11345096587559468No Hit
AAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG185480.11153275640327184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT183280.11020985331891128No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG182720.10987311435198313No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC181340.10904329332633879Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC180370.10846001333005253No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA180240.1083818417841585No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT179330.10783464096290026No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG176420.10608480097404152No Hit
ACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCC173900.10456947562286487Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG172980.10401626160576863No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG168290.10119607275774542No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG167970.10100365049092934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)