FastQCFastQC Report
Thu 28 Sept 2023
SRR6181551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24374055
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2444471.0028983687777844Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2329580.955762182369737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2232420.9159001241278892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1855270.7611659200736193No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1804510.7403404973033826No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1346820.5525629609024842Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1086910.4459290831993281Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA982680.4031663996819569Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG973290.39931394263285286TruSeq Adapter, Index 4 (100% over 49bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG970490.39816518014749697Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC765780.31417833429849895Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC533580.21891310247720372Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC518000.21252105979083086Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC511980.2100512204473158Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC500420.20530847247206097Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG490310.20116061935529397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC483070.19819024778601674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC474540.1946906249288434Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC468280.19212232022944067Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA466390.1913469055518255Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC399990.16410482375624408No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA398460.16347710711246036No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT390100.16004723054904077No Hit
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG386370.1585169148096203TruSeq Adapter, Index 4 (100% over 35bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA383160.1571999406746231Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC373920.1534090244729488Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC373670.15330645639389917Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT364910.14971247090400017Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG358320.14700877634025195No Hit
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC338750.138979747112247Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA333320.13675196843528908Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGACGAGGCGCCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA333300.1367437629889651Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCGCGCGCCGCGGCTGGACAGATCGGAAGAGCACACGTCTGAACTCC324390.13308823665163635Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA319080.13090969065262223Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC317600.13030248762464844Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG307750.12626130531009305No Hit
TTCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC303690.12459559970632707Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC282830.11603731919042604Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC259420.10643284426821881Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA254800.10453738616738167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA250520.10278142065405203No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG245520.10073005907305944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA245050.10053723108444615Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)