FastQCFastQC Report
Thu 28 Sept 2023
SRR6181552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23080557
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2330701.009810985064182Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2199180.9528279581814252Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2114180.9160004240798868Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1747260.7570267909912226No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1668700.7229894841792597No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1283370.5560394404693093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT971110.4207480781334696Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG867010.3756451804867621TruSeq Adapter, Index 4 (100% over 49bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG855140.370502323665759Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA843540.3654764484236667Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC673460.29178671901202385Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC488340.21158068238994404Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC473730.20525067917555023Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC471170.2041415205014333Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG458920.19883402293974103Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA450020.19497796348675642Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC439150.19026837177283026Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC435370.1886306296680795Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC419020.18154674516737182Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC414300.17950173386196874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT361860.156781311646855No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC361300.1565386831868919No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA354780.15371379468875035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA348880.15115753055699652No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC348280.15089757149275038Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG345860.14984906993362423No Hit
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG342740.14849728279954422TruSeq Adapter, Index 4 (100% over 35bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT341450.147938370811415Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC336860.1459496839699319Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGACGAGGCGCCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA319600.13847152822178424Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCGCGCGCCGCGGCTGGACAGATCGGAAGAGCACACGTCTGAACTCC314940.1364525128228058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC310790.13465446262843656Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA305280.13226717188844272Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC305140.13220651477345197Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA303260.13139197637214733Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC291070.12611047471687967Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG273720.11859332510909507No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC250770.10864989090167972Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA242610.10511444762793204Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA238620.10338571985069511Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC237990.10311276283323666Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA234840.10174797774594435Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA232990.10094643729785203No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG231730.10040052326293512Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)