FastQCFastQC Report
Thu 28 Sept 2023
SRR6181553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24389745
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2472851.0138892391043859Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2304040.944675723341921Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2191080.8983611759778546Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1843910.756018564359734No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1838520.7538086191553048No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1337630.5484395183303474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1073900.440308006500273Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA989210.4055843962288249Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG968660.397158723881697TruSeq Adapter, Index 4 (100% over 49bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG964120.39529728580598117Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC758870.31114306443138295Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC551290.22603352351572353Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC539710.22128562639748797Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC523220.2145245881004496Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC502040.20584061046968716Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC489760.2008057074807465Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG478580.1962218137171996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC471730.19341325626815697Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA470620.19295814695889604Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC468100.19192492582435774Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC411370.1686651500456442No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA405680.16633220232519857No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT401480.16461016710096804No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC378680.1552619758837167Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG374250.15344563873054023TruSeq Adapter, Index 4 (100% over 35bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC373600.1531791332791712Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA370920.15208031080275747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT368420.1510552898359536Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG358660.14705360798155126No Hit
GGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA345240.14155129543174805Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGACGAGGCGCCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA340270.13951355374974195Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC331520.1359259803659284Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGCGCGCGCCGCGGCTGGACAGATCGGAAGAGCACACGTCTGAACTCC331020.13572097617256762Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA324950.1332322252651678Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC323490.13263361302055435Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG319260.13089927754472216No Hit
TTCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC311790.1278365148959122Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC284300.11656538434493678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC259660.10646277769611777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA257900.10574116293548784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA257690.10565506117427632No Hit
TCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA252490.10352301756332426Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG250900.10287110422843698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA250030.10251439693198924Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)