FastQCFastQC Report
Thu 28 Sept 2023
SRR6181554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24764420
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2527431.020589216303067Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2434640.9831201376813994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2342840.9460508261449289Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1934030.7809712482666664No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1864590.7529310195837415No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1411150.5698296184606787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1156130.46685123253441835Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1044040.4215887147770874Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1007340.4067690662652305Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG997650.4028561944919364TruSeq Adapter, Index 4 (100% over 49bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC784270.31669225445215354Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC563020.22735036798762095Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC552740.22319925118375475Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC543240.21936310238640758Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC510180.20601330457163947Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG507950.2051128191171043Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA489510.19766665239888517Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC487470.19684288992029694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC472600.19083830753960723Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC471050.1902124095779348Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA404980.1635330042052267No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT401380.1620793057136004No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA391000.1578878083960779Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC389110.15712461668797412Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG387630.15652698508586108TruSeq Adapter, Index 4 (100% over 35bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC386480.1560626091788138No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC382670.15452411160850932Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT382150.15431413293749663Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG369770.14931502534684843No Hit
TGGACGAGGCGCCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA350210.1414165968756789Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC349480.141121819125988Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA340910.1376612091056443Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCGCGCGCCGCGGCTGGACAGATCGGAAGAGCACACGTCTGAACTCC339450.13707165360626253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA334960.1352585685430953Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC329000.13285188992918065Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC326290.1317575780090953Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG318020.12841810952972046No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC288610.11654220046340678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA268970.10861146758131222Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA265880.10736370970933298Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC265630.10726275842519227Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA261450.10557485295435952No Hit
TCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA257180.10385060502123612Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG250880.10130663266089009Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)