FastQCFastQC Report
Thu 28 Sept 2023
SRR6181555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17293144
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2025501.1712734248902341Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1916251.1080980994549052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1913621.1065772655336705Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1614210.9334392866907255No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1338510.7740119436928299No Hit
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1171400.6773782719903333Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1167370.6750478686813688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1051980.6083220032169975Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT839710.48557393612173705Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC790120.45689783188065747Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG769640.44505498826587003TruSeq Adapter, Index 4 (100% over 49bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC704940.40764131727579433Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC431240.24937050197465538Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC419830.24277251146465909Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG386510.2235047600366943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC362730.2097536457222585Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC350360.202600521917819Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC342460.19803223751563045Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC342000.19776623614537647Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA331910.19193155391523947Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA313140.1810775414811789No Hit
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG307400.1777583069914875TruSeq Adapter, Index 4 (100% over 35bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC302710.1750462495425933No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA302200.17475133497992038Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT293220.1695585256214833No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC279320.161520658129025Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC276290.15976851866843877Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT273630.1582303368317525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC273200.15798168337694984Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG255350.1476596736833973No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC249140.14406865518496811Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG245830.14215460184683595No Hit
TGGGCGCGCGCCGCGGCTGGACAGATCGGAAGAGCACACGTCTGAACTCC243380.14073785541830913Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGACGAGGCGCCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA241570.13969119785274442Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA236790.13692709665749617Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC232360.13436538780918034Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA228770.13228942059350227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGACGAGGCGCCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC224970.13009201796966474Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGAGGCCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC197280.11407989200806978Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC193690.1120039247923917Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG192030.11104400680408373Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA189040.10931499789743264No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA187120.10820473130854633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA185620.10733733553597888Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTGCGACCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA184580.10673594113366545Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG184160.10649307031734657No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA179300.10368270801422808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT179140.10359018579848753No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC177240.10249148448656878No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)