FastQCFastQC Report
Thu 28 Sept 2023
SRR6181556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22708294
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3342241.471814659436768Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3017511.3288140447714831TruSeq Adapter, Index 10 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2455311.0812393040181707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2091990.9212448984498791No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2046410.9011729370775277Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1866310.8218627079603602No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1676420.738241278715169No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1586580.6986786413809861No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1320970.5817125672232357Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1158600.5102100580519171No Hit
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1068340.47046246626893234Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1049330.46209107562197316No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1015660.44726389397635946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT995520.43839488778857627No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA927840.40859079946736643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC872360.3841591975161146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG847690.373295325487683No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG809310.35639401180907737No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG749770.33017451685274113No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG680540.29968785854190544No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG662080.29155867014932957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT657620.28959463005014824Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC655030.2884540776158702No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA645620.28431021722723865No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG611970.2694918429363298No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA593160.26120852583641907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG581830.256219159396122No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG573620.25260374028978133No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA532420.2344605895977919No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC515660.22708002635512822No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG490230.2158814748479124No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA463610.20415888573575802No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG456120.200860531398792No Hit
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC453390.19965832748158008Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC438590.19314088499999163Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC436710.1923129936577358Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG432260.19035335723590682No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA431000.18979849388950135Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC420580.1852098620882749No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT417550.18387554785049023No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG415960.1831753631514547Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT400850.1765214066719411No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA393140.1731261714332217No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA390960.17216616977039315Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC372640.16409863286075124Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC369320.16263661197974624No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT365970.161161380066684Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC357780.15755476831504825No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG354660.15618082098109176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA354040.15590779298524143Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAA348910.15364870650344759Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC334300.1472149338915552No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG333960.1470652088615728No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG329120.14493382902299928No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT329110.14492942534564685No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT326710.14387254278106493No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT319160.1405477663799843No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG311720.1372714304297804No Hit
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA302420.13317601049202551Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT295580.1301638951829671Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA287300.1265176503351595Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC280660.12359360857314952No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA275450.12129929267253631No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG263430.1160060724949219No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC262340.11552607166350762No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT261440.11512974070178938No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG256120.11278698435029949No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCAC254260.11196790036274852No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC252720.11128973405047513No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC248810.1095678962056771No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT248310.10934771233805586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC244910.10785046203823148Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA243160.10707981850155718No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC240240.10579394471464919No Hit
ACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACT239360.10540642110763582Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA228640.10068567898583662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT228130.10046109144086297No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC227870.10034659582969993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)