FastQCFastQC Report
Thu 28 Sept 2023
SRR6181558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22825596
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3320091.454546904273606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3027681.3264407203211692TruSeq Adapter, Index 10 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2411701.0565770111764006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2077740.9102675785552324No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2027830.8884017749196997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1928230.8447665506740766No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1708620.7485543860497662No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1625630.7121960802250246No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1334340.5845805734930207Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1190460.5215460748538615No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1083540.4747039244889816No Hit
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1069240.46843902783524255Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1026510.44971881566641236No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1025170.4491317554205375Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA942430.41288297576107097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC886460.3883622578792685Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG857980.3758850371311225No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG830420.36381087267118895No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG777190.34049056156080215No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG701780.3074530890672033No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC676440.29635151695491324No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA668690.2929562058313834No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT662110.29007347716134113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG655140.28701988767346975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG622900.27289539339958524No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG593980.26022540660055493No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC587700.2574741093288429No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA580940.25451252181980266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG571740.2504819589376768No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA544440.2385217016896295No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG537260.2353761102229269No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA478170.20948850579849046No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG465320.20385886090334726No Hit
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC465210.2038106693906262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC447680.1961306946815321Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC446910.1957933540924846Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG442940.19405407858791507No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA439440.19252071227406287Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC433060.1897256045362408No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT425570.18644420062459705No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG412160.18056921711923754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT410340.1797718666360344No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA407350.1784619336993435No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA389680.17072062433769528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC383310.16792989764648425No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC378890.16599347504441941Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC370740.16242292205644926No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT369100.16170443041224422Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT360830.15808130486494196No Hit
CTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAA359610.1575468171783992Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG354780.15543077166528313Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA353660.15494009444485043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC350030.1533497745250551No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG347270.15214060566041737No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG340680.14925349594376416No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT329880.14452196560387734No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG323520.1417356199592773No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT314550.13780582114920462No Hit
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA310950.1362286443692423Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT303850.1331181012754278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC289000.12661224705808338No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA288280.12629681170209095Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA282580.12379961513381732No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT275730.12079859820527798No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG272600.11942733061603297No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT270640.11856864548027574Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC265790.11644383787393765No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG259760.11380206676750083No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCAC259380.11363558699628258No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC257330.11273747244102629No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC256810.11250965801725397No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC247890.1086017644402363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACA247650.10849661932157216No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA245470.10754155116037276No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT243630.1067354385839476No Hit
ACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACT243220.10655581567289635Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC242040.1060388521727976No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC237190.10391404456645952No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA230330.10090864659130915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAGATCGGAAGAGCACAC228810.10024272750643619No Hit
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC228330.10003243726910789Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)