FastQCFastQC Report
Thu 28 Sept 2023
SRR6181569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24444609
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG7413053.0325909487854763TruSeq Adapter, Index 12 (100% over 49bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3103641.2696623619547363Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2147530.8785290859019264No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1819230.7442254445550754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1688080.6905735330027165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1577670.64540610978887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1553310.635440722328592No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1104340.4517724132957087No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA972510.39784232179782464No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC842260.344558589585131No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC832370.34051270773036296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT615490.2517896686340943No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA580520.23748385584731585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC568140.23241934448614007Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACT565240.23123298883610696Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCC526140.2152376419684193Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA490180.20052683190800885Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA475770.19463187159180986No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC474050.1939282399648937Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA466160.19070053442049328Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG465120.1902750827391021Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG461120.1886387301183668No Hit
ACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT451560.1847278473548094Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT445750.18235104517319137Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC445530.18226104577905092Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG443010.18123014362798767Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA437320.17890243202499168No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG435110.17799834720203542No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATG430320.17603881493870488TruSeq Adapter, Index 12 (97% over 49bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT420780.17213611393825115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG414560.16959158561300774No Hit
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA384620.15734348624680394Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG383630.15693848897317195No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG382030.15628394792487785No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG362020.14809809393964946No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT360540.14749264346997737No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT355580.1454635662202656No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC353000.1444081187798913Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT345080.1411681405908354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA343250.14041950926684899No Hit
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC342920.14028451017563834Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA326640.1336245550092456Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA315880.12922276645946762No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA315200.1289445865139426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC302340.12368371283827857Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT301020.12314371647343592No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC294160.12033737172887486No Hit
ACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAA288900.11818556803260792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACT285970.11698693973791932Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG285340.1167292142001535No Hit
CTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACTCC282170.11543240474822077Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG275590.11274060468711117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG273620.11193470102139903No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA272070.1113006143808641Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG270310.11058061922774055No Hit
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC269720.11033925721618211Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA268420.10980744261444313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACCCGCAACGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC267990.10963153470771407Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACT265510.1086169960828582Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC264480.10819563528301884No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT250340.10241112876871951No Hit
AAGACCCGCAACGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC250180.1023456746638901Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC246010.10063977705677354Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)