FastQCFastQC Report
Thu 28 Sept 2023
SRR6181570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25705963
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG8206483.192442158264991TruSeq Adapter, Index 12 (100% over 49bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3326641.2941121871217196Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2372270.9228481344970426No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1881400.7318924406761187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1754850.6826626180081252Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1700960.6616986105519563No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1661020.6461613595258034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1248130.48554103964126916No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1034230.40233077438102594No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC930320.36190824673637007Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC884050.3439085320398228No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT679920.26449894135457985No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA616140.23968757754766862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACT611480.23787476858968482Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC605780.2356573842419364Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCC568510.22115880272604452Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA546620.2126432688010949No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC530840.20650461529101244Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG526580.20484741225216888Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA511990.19917168635152863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG502040.19530098911291516No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG497920.1936982481457707No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA494450.1923483667972291Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT484710.18855936266616427Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA482700.18777744292248455No Hit
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC477220.18564564183026327Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT473620.18424518855800112Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG465090.1809268923323355No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG461130.17938639373284712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT454610.17685001725086122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG426960.16609375809029212No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT410220.15958165037427308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA405690.15781941334000987Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG405200.15762879608906308No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT402290.15649676302731783No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG401150.15605328615776815No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT379710.1477128088918513No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC377870.14699702166380618Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATG373500.14529702699719904TruSeq Adapter, Index 12 (97% over 49bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA371280.14443341414597072No Hit
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC364970.14197873077153345Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA356970.13886661238872866Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA353360.13746226896848798Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT350660.13641192901429136No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA343420.133595461877853No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC336660.13096572184438296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG316230.1230181495242952No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC315860.12287421404909048No Hit
AGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACT310000.12059458733368596Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA308940.12018223164796432Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAA307710.11970374344660809Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACTCC299810.11663052654358834Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG295890.115105588536014No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG295290.11487217965730363No Hit
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC292460.11377126777938644Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACT292210.1136740140799238Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA292210.1136740140799238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG287850.11197790956129518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC284050.11049965332946289No Hit
AAGACCCGCAACGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC283610.11032848681840864Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT278200.10822391676203688No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG277390.1079088147757779No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG269690.10491340083232828No Hit
AAGACCCGCAACGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC267200.10394475398568029Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC267140.10392141309780924Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC263360.10245093716193397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)