FastQCFastQC Report
Thu 28 Sept 2023
SRR6181571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26088295
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG8439993.235163509152285TruSeq Adapter, Index 12 (100% over 49bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3373031.292928495327119Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2405310.9219881943223963No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1995230.7647989261084329Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1861350.7134808924845414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1754500.6725238272566298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1720420.6594604975143068No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1176660.45102985840968146No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1050560.4026940051084212No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC932820.3575626540561581No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC930160.35654303970420453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT665280.25501091581492774No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA646480.24780461889134572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACT620800.2379611239446656Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC611190.23427747961298354Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCC571930.21922858508001386Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA551730.21148564902382466No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG547410.2098297339860654Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC543450.2083118118681194Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA534100.20472782908963577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA507780.19463901339662099Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG505820.19388771861097093No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG499150.1913310164577639No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG493430.18913846228739747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT492850.18891614036103166Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT488770.18735222060314788Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC488090.18709156731016727Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA486790.18659325954417488No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG442710.16969679314037195No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT439670.1685315195952821Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG423290.1622528417437782No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG422250.1618541955309843No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG415070.15910200340804181No Hit
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA411430.15770674166326315Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT402630.15433358140116094No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC393500.1508339276292299Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT391240.14996763874373545No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATG378310.14501139304044208TruSeq Adapter, Index 12 (97% over 49bp)
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC376220.1442102674781928Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT373740.14325964958614582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA372510.14278817377678382No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA372270.14269617849690827Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA367070.1407029474329388Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC345330.13236970833088174Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA344590.13208605621793224No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT325220.12466127050464586No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC320660.12291336018701107No Hit
AGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACT319680.12253771279418606Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAA318300.12200873993490184Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA315740.121027456949563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACTCC314400.12051381663692473Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG311100.11924888153863639No Hit
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC303640.11638936158917246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACT302200.11583738990991936Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG300130.11504393062099305No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG299150.11466828322816804No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG298430.1143922973885415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC298420.11438846425188001No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA295030.11308903092363835Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACCCGCAACGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC288800.11070098678353645Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC280200.10740448925466382Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG276790.10609738965309923No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT276490.10598239555325481No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG267900.10268973116104368No Hit
AAGACCCGCAACGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC266460.10213775948179059Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT262950.10079232851361117Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)