FastQCFastQC Report
Thu 28 Sept 2023
SRR6181572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48896000
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG16585933.3920831969895286TruSeq Adapter, Index 12 (100% over 49bp)
AAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6267001.281699934554974Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4654080.9518324607329842No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4433440.9067081151832461Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4095960.8376881544502618Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3939710.80573257526178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3404510.6962757689790576No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC2461150.5033438318062827No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2083150.42603689463350786No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1886390.38579638416230366No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1748810.3576591132198953Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1529460.3127985929319372Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT1367250.27962410013089006No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1348810.2758528304973822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACT1262140.25812745418848165Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1214530.24839046138743454Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAACTCC1174730.2402507362565445Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1151730.235546875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1142460.23365101439790573No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1055580.21588268979057593Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1053500.21545729712041886No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1015340.2076529777486911No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1011370.20684105039267017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT977600.19993455497382198Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACT960650.1964680137434555Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA949720.19423265706806284No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG948910.19406699934554975No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA940010.1922468095549738Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC935490.1913223985602094Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG910890.18629131217277486No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT906280.1853484947643979Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT875990.17915371400523558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG864560.1768160994764398No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT844080.17262761780104713No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT831550.17006503599476439No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC808430.16533663285340314Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA786090.1607677519633508No Hit
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA774330.1583626472513089Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA770380.15755481020942408Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG756010.1546159195026178No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC742750.1519040412303665Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT722320.14772578534031414No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA704300.1440404123036649Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT703280.1438318062827225No Hit
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC699580.14307509816753927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC677920.13864528795811518No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA669560.13693553664921465No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA665760.13615837696335079Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG650990.13313767997382198No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG644580.13182673429319372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACT632620.1293807264397906Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAA627280.12828861256544502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGACCCGCAACGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC623610.12753803992146595Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCAGATCGGAAGAG619730.12674451897905759No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA615550.12588964332460734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGAGATCGGAAGAGCACAC613930.12555832787958116No Hit
AGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACT611900.12514316099476439Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC609740.12470140706806283No Hit
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC599310.12256830824607329Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACTCC593560.12139234293193717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTC592560.1211878272251309Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG588920.12044339005235602No Hit
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC583270.11928787630890053Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG572940.11717522905759163No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG564300.11540821335078535No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC560250.1145799247382199No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT559980.1145247054973822Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGACCCGCAACGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC537300.10988628926701571Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT526920.10776341623036649No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG520990.10655063808900522No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT510740.10445435209424084No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT501210.10250531740837697Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC497620.10177110602094241TruSeq Adapter, Index 12 (100% over 50bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA496340.10150932591623037No Hit
AAGACCCGCAACGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC491840.10058900523560209Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)