FastQCFastQC Report
Thu 28 Sept 2023
SRR6181574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6181574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29035853
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC8396422.8917421506438954No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6422092.2117793474157623No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT5090771.7532703447699642No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4971931.7123416350124103No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3875051.334574190053931No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3617941.2460250435900746No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2496090.8596578857180466No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2418700.8330046305166237No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2342360.8067129972038363No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2273570.7830215974712366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC2251670.7754791980796982No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2097080.7222381240186055Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2093790.7211050421008813Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1831250.630685793870082Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1606450.5532642695222352No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1524220.5249441096151024TruSeq Adapter, Index 10 (100% over 49bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1475800.5082681745220298No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1433250.49361387798732825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1337340.4605823014739743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1143670.3938820051196705No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1118250.3851273113967067No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1097090.3778397693362065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1044910.3598688834800204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAG1014790.34949550130316476No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT975490.33596051061423954No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC936130.3224048558173924Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA909690.31329887225975417No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT830020.28586038095729444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG758670.26128731262002186No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA687180.23666602803093126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGC679000.23384882131756213No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC657220.22634775014186773No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC650250.223947269604926No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC644660.22202206355019086No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT614110.21150058859989407No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG607930.2093721854839257No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG606200.208776370372174No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG560580.1930647603154624No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA552550.1902992138718983No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC543480.18717548955768581No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG512170.1763922692403767No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA512050.17635094102453266No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT511510.17616496405323445No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG503850.1735268462751895No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA499120.17189782576733667No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA489610.168622564661696No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGA458860.15803220935165913No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG446760.15386494758738448No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT444170.15297294692875046No Hit
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC411840.14183843677676697Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT408350.14063647449930264No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT397960.13705813981080564No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTAGATCGGAAGAG349170.12025477605221378No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC340970.11743068130287061No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT314480.10830747765529741Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT313830.10808361648614216No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCAGATCGGAAGAGCAC309610.10663024089562652No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGAGATCGGAAGAGCA308060.10609641810764092No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG304340.10481524341647548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGC298800.10290725745167534No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC296840.10223222992622259No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCAGATCGGAAGAGCA291050.10023814351174737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)