FastQCFastQC Report
Tue 27 Jun 2023
SRR618770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR618770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9742062
Total Bases467.6 Mbp
Sequences flagged as poor quality0
Sequence length48
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAA1351421.3872011900560681No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAA1161901.1926633191207365No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA673940.6917837312059808No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA631220.6479326450601526No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA605680.621716429232333No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA581260.5966498673484114No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAA557610.5723736925509199No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA498510.5117089174755817No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA457730.4698491961968626No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA434830.4463428789510886No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA353220.3625721125568694No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCAAAAAAAAAAAAAAAAAAA334030.34287402400025785No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAA332520.34132404412946665No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAAAAAAA301090.30906188032882564No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAA287390.29499914905078617No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA285530.2930899023225268No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATC271680.2788731995341438No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAA271280.27846260883989443No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAA223660.22958178668951193No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAA202530.20789233326579115No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA201570.2069069155995928No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA196990.20220565215043795No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAA196650.20185665006032605No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAA190400.19544117046268028No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATCG186550.1914892350305305No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA183250.18810186180297353No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAA170090.17459342796217064No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA164290.16863986289555538No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA156020.16015090029195053No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAAA153410.15747179601197364No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAAAAAA149430.15338641860419283No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA142600.14637558249988555No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAA142380.1461497576180484No Hit
TTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAAAAAA141160.14489745600058795No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCCAAAAAAAAAAAAAAAAAA137520.14116108068291908No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA127970.13135822785771636No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAAAAAA124390.1276834411441849No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAA124090.12737549812349788No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAA114190.117213378440827No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA106770.10959692106250196No Hit
TCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAAAAAAAAA106600.10942242001744598No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAA105790.10859097386159113No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA104900.10767740956688636No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA102320.10502909958897819No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAAAAAAA101920.10461850889472886No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAAAAAAA99100.10172384450027108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)