FastQCFastQC Report
Tue 27 Jun 2023
SRR618771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR618771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44827310
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length48
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAA5707711.2732662298942319No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAA5439671.2134723230102364No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA3476300.7754870858858138No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA3395370.7574333592624675No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA3214900.7171744188977657No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA2953970.6589665987095813No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAA2646700.590421330211427No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA2447390.5459595947202721No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA2310060.5153242521132765No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA2172080.4845439086128523No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAA2106470.4699077415084688No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1729960.3859165316857068No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAA1627410.3630398522686282No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAA1560370.34808468319870184No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA1508450.33650245798822187No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAA1340670.2990743812198412No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCAAAAAAAAAAAAAAAAAAA1314690.2932788070486496No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAAAAAAA1268700.2830194361428335No Hit
CCGCCGAGGGCGCACCACCGGCCCGTGTCAAAAAAAAAAAAAAAAAAA1167520.26044837399344284No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATC1078330.24055202063206557No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAA1053380.23498621710738385No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA1028450.22942487514865378No Hit
ATTAAATCAGTTATGGTTCCTTTGGTGGCTAAAAAAAAAAAAAAAAAA1024290.22849686943071087No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAA999780.22302922035696543No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA965780.21544455823916267No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA925560.20647234911039722No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAA923330.20597488450678839No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA907850.20252163246021232No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAA857950.19139002541084887No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA841800.18778731090489256No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAAA805250.17963379912825464No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGGGGCAAAAAAAAAAAAAAAAAA665810.1485277613133601No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA661220.14750383192745672No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATCG658600.14691936678779074No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA642370.14329880601802786No Hit
TTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAAAAAA640730.14293295760999267No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAA638210.14237080030008492No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGGGGCCAAAAAAAAAAAAAAAAA617060.13765269430621646No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAA615160.13722884554080983No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAAAAAA593340.13236127708756112No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA569370.12701409029451022No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA566090.12628239347843984No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAAAAAA558210.12452453649349024No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAA543680.12128320882961749No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAC536010.1195721982871602No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA531790.11863080787136233No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCCAAAAAAAAAAAAAAAAAA529060.11802180411896229No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAA521590.11635540923602151No Hit
TCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAAAAAAAAA520030.11600740709179291No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA513480.11454624424262799No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAT504940.11264115558127401No Hit
CCGCCGAGGGCGCACCACCGGCCCGTGTCGCAAAAAAAAAAAAAAAAA503420.11230207656894871No Hit
TTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAAAAAAA482500.1076352785835242No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAAAAAAA468480.10450772085141848No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAA468180.10444079736214373No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAA451240.10066185100109733No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)