FastQCFastQC Report
Tue 27 Jun 2023
SRR618772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR618772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15628782
Total Bases750.1 Mbp
Sequences flagged as poor quality0
Sequence length48
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA2238001.4319733937040007No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA2000831.280221324988729No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA1029230.6585477998221486No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA1002710.6415791070602942No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAA994720.6364667444974279No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAA720920.46127714878869003No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAA671280.4295152366959882No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA653890.4183883299415143No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA615540.39385026933000916No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAAAAA471220.3015078206350309No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAA447010.2860171701160078No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA394450.252386910253147No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA376500.2409016902276838No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAA364930.2334986821109924No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAAA342780.21932611255310874No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAAAAAAA303450.19416100371737222No Hit
TTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAAAAAA291160.18629730710940878No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA290420.18582382171560138No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA287660.18405784916572515No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAAAAAA284430.1819911494062685No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAA282600.18082023282428536No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA277900.1778129607284816No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA265280.16973811522868512No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA261190.1671211486602091No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAAAAAAA257240.1645937604094804No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA254810.16303893675143719No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGAAAAAAAAAAAAAAAAAAA247450.15832967661843386No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA246820.1579265741885708No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA241970.15482332532375204No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAT233410.1493462510386286No Hit
TCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAAAAAAAAA230970.14778502892931772No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAAAAAA225910.14454741258787793No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAAAAAAAAAAA216860.1387568141906388No Hit
GATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAAAAAA211200.13513529077313896No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAAAA207480.1327550669015666No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAA200180.12808419747616928No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAAAA198690.12713082823728683No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA192990.12348371101471631No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA190600.12195448116174376No Hit
AGTTAAAAAGCTCGTAGTTGGATCTTGGGAAAAAAAAAAAAAAAAAAA185360.11860169269748595No Hit
TTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAAA184630.11813460575494622No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAAAAAAA184270.1179042615093102No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCAAAAAAAAAAAAAAAAAAA178730.11435951950702238No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA175410.11223523368615673No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAAAAT172760.11053964410022482No Hit
TTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAAAAAAA168260.10766034102977443No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAAAAAAATC167470.10715486337962868No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA166760.10670057333962428No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAAAAA163590.10467226428777367No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAAAAAA159470.10203610236549462No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAA158970.10171617980211127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)