FastQCFastQC Report
Tue 27 Jun 2023
SRR618773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR618773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28457106
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length48
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA3846241.351592112001832No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA3348541.176697307168199No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAA2213780.7779357465232059No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1800800.632812064585907No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA1765250.6203195785263617No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAA1763460.6196905616474142No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA1752660.6158953760090713No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAA1669810.5867813824778949No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA1102620.38746736931014697No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAAAAA996100.3500355939216026No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAA922970.32433726746493474No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA849090.29837538645004874No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAA829700.2915616226049128No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGGGGCAAAAAAAAAAAAAAAAAA817000.2870987654190837No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGGGGCCAAAAAAAAAAAAAAAAA719730.2529174962485644No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAA715270.2513502251423599No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA706630.2483140766316856No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA669050.235108236234563No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA630520.22156856006369727No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA610720.2146107197267354No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA592600.2082432416001824No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA583490.20504193223302467No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAAA555410.1951744495733333No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAAAAAAA549990.1932698286326094No Hit
TTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAAAAAA535030.18801279371134927No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAT522820.18372212550355613No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA506190.1778782424326634No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAAAAAAA501840.1763496259949975No Hit
TCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAAAAAAAAAA500160.17575926378458864No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAAAAAA496760.1745644831206659No Hit
GATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAAAAAA493330.17335916027441442No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA483380.16986266980205225No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA479130.1683691939721488No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGAAAAAAAAAAAAAAAAAAA472430.16601477325206576No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA460790.16192440650851847No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAA429730.15100973373750656No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAAAAAAAAAAA420140.14763974945308914No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAA417960.1468736842038681No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA414350.14560510826364423No Hit
TTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAAAAAAA392150.13780389334038395No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA385470.13545650074185336No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAAAA382910.13455690118313507No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAAAAAA381490.1340579045529085No Hit
TTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAAA375510.13195649620871497No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGGGACAAAAAAAAAAAAAAAAAA373730.13133099339054363No Hit
AGTTAAAAAGCTCGTAGTTGGATCTTGGGAAAAAAAAAAAAAAAAAAA368730.12957396300242197No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA363310.12766934206169805No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAAAA341500.12000517550871126No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAA338990.11912314625387417No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAAAAAA336230.118153265479631No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCAAAAAAAAAAAAAAAAAAA332300.11677223959456735No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAAAAAAA327040.11492384362626333No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAA324760.11412263776927985No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAA314590.11054883795984033No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAAAAT303810.10676068044304997No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA301200.10584351058045044No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAAAAA298660.10495093914328463No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAAAAAAA295600.10387563654575417No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA294570.1035136882858011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)