FastQCFastQC Report
Mon 26 Jun 2023
SRR618774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR618774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25957180
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length48
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA3918391.5095592048134658No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAA3129821.2057627215283018No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA2832641.0912741676869369No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA1700430.6550904219949933No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA1219450.4697929436094368No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA1174410.4524412898473563No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA1091700.42057727380247No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA846540.32612941775647436No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAA846490.32611015526339915No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA836320.322192164171917No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATG802470.3091514563600514No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA780110.30053726945685166No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA721170.27783064261988394No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAA672310.2590073343868633No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATCG581560.22404590945549557No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATC531410.20472562890113644No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAT521490.20090395027502986No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA438110.16878181682293686No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAAAAAA412610.15895794535461866No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAAT411780.15863818796957144No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAA401210.15456609693348816No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA381860.1471115121134114No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATGG337110.1298715808111667No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCCAAAAAAAAAAAAAAAAAA336870.12977912084440607No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA333540.12849623880560215No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAAAAAAAAAA326050.1256107173429471No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA310100.11946598205197945No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAAAAAAAA306690.11815228002425533No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAAA286180.11025080536483547No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAAAAAAAA285330.10992334298255821No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAAAAA283770.10932235319861403No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCAAAAAAAAAAAAAAAAAAA283230.10911431827340258No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA281490.10844398351438791No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA274470.10573952948663916No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAATCGT274040.10557387204619299No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAAAAA268440.10341647282177803No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGGAAAAAAAAAAAAAAAAA265520.10229154322619022No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAT263470.10150178101010973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)