FastQCFastQC Report
Tue 27 Jun 2023
SRR618775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR618775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43068755
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length48
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA7215901.6754373327020944No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA6908211.6039957505156581No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA4397981.0211532699285133No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA4042530.9386224421857563No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAA2492270.5786724041593494No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA2286750.5309533558608788No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1555300.36112025991928487No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAAAAAAAAA1410190.327427621253505No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA1400060.3250755681235736No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA1321750.30689301327609775No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAAAAA1071890.24887879856290251No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA936980.21755446610889959No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA925370.214858776391377No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA920430.21371177318684972No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA894590.2077120641170148No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA869910.20198169183204853No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAAAAAA746150.17324624312915476No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA735920.1708709713108726No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA722250.16769697661332444No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAAAAAAA687530.15963544801794247No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA611690.14202639477273027No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAAAAAA590160.1370274111708128No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAA588630.13667216523904627No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA588440.13662804973117984No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAA565040.13119487665710328No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAAAAA485090.11263153532067505No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAAAAAAAT470120.10915569767456709No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA469440.10899781059378197No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAAAAAA453600.10531997035902246No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAA452110.1049740119025962No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA445800.10350891266766361No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAA436900.10144244940444645No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAAAAA436790.10141690884726062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)