FastQCFastQC Report
Tue 4 Jul 2023
SRR6202814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6202814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16824093
Total Bases478.1 Mbp
Sequences flagged as poor quality0
Sequence length24-48
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGCACCCAACTTACACTTAGGAGATT3762152.2361680953618124No Hit
AGCTTGGGCTGCAGGTCGACCCGT2554801.5185365416132686No Hit
TGGAGCTTTAATTTATTAATGCAAACAG2363751.4049791569744652No Hit
AAGCACCCAACTTACACTTAGGAGATT2288551.3602813536515757No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCGT1132500.6731417854145243No Hit
CGACTCTTAGCGGTGGATCACTCGGCTC861120.5118373989016822No Hit
AGGTTGCGGGTTCGACTCCTGCTGGGTA798970.47489632873522514No Hit
AGGCGAGCGAGAAAACTAGTGTTAAGGAAC717160.42626963605110835No Hit
GCAATAAACGAAAGTTTAACTAAGCTAT649420.3860059499195588No Hit
CTTAACACAAAGCACCCAACTTACACTT633380.37647200357249566No Hit
TTAACACAAAGCACCCAACTTACACTT597380.3550741190030274No Hit
AATACAAGCTTGGGCTGCAGGTCGACC517520.3076064783997568No Hit
TGGAGCTTTAATTTATTAATGCAAACAGT494540.2939474954162462No Hit
GGCATCAATATGGTGACCTCCCGGGAGCGG484080.2877302211774507No Hit
AATACAAGCTTGGGCTGCAGGTCGAC469430.2790224709290421No Hit
ACTGTCTCAACACTAGACTCGGTGAAATT434820.2584507824582282No Hit
GGAGCATAGCTGGGTAGCTAAGTACGGAA411720.24472047319281937No Hit
AGGCGAGCGAGAAAACTAGTGTTAAGGAA407730.2423488743197033No Hit
AACCTGCATTAAAAATTTCGGTTGGG400220.2378850378442392No Hit
TGGAGCTTTAATTTATTAATGCAAAC391600.2327614332612165No Hit
CTGTCTCAACACTAGACTCGGTGAAATT340230.2022278407519502No Hit
AAAGCACCCAACTTACACTTAGGAGATT327590.1947148057253369No Hit
AGGTTGCGGGTTCGACTCCTGCTGGGT315500.18752868282409044No Hit
GGCGAGCGAGAAAACTAGTGTTAAGGAAC309730.18409907743615064No Hit
GGCGAGCGAGAAAACTAGTGTTAAGGAA308060.1831064533464003No Hit
GGTCCCTATTTAAGGAACAAGTGATT300250.17846430116619066No Hit
GGGGTTTTAGTTAAATGTCCTTTGAAGT294350.1749574256395278No Hit
TAGTACCGCAAGGGAAAGATGAAAAATT290260.17252638819816318No Hit
CAAGCTTGGGCTGCAGGTCGACCCGT287850.1710939187033738No Hit
CTTAACACAAAGCACCCAACTTAC263360.15653741333931048No Hit
GAGATTCCTTATAGACTATGAGGTTGAT255930.1521211277184452No Hit
CTGGCAGGATCAACCAGGTAGGTAGGC244600.14538673793588755No Hit
GGAGAGTTCTTGGGTGGGTGTGGGTAT240850.14315779162656792No Hit
TACGACTCTTAGCGGTGGATCACTCGGCT232970.1384740324485843No Hit
GGTCCCTAATTAAGGAACAAGTGATT230180.13681569639445051No Hit
CGACTCTTAGCGGTGGATCACTCGGCT216390.12861911783297916No Hit
TGAAGCACCGCCAGGTCCTTTGAGTTTT215100.12785236030257321No Hit
GAGAGAGTAAAAAATTTAACACCCATAGT199120.11835407709645923No Hit
CTGCTAAACCGCAAGGTGATGTATAGGGG194270.11547130653640585No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGT193970.1152929908316603No Hit
CTAAGAGCTAATAGAAAGGCTAGGACC192780.11458567186950286No Hit
TGCTCATAAGGAAAGGTTAAAAAAAGT189630.11271335696967438No Hit
AATCACCCAACTTACACTTAGGAGATT186080.1106032877968518No Hit
CGCATGAAGATTACGGAAAAGAAGCGT182170.10827923977833455No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTC180510.10729255954540907No Hit
ACAGGTTAATATTCCTGTACTTTCTA179410.10663873529467532No Hit
GGAGCATAGCTGGGTAGCTAAGTACGGAAA177050.10523598508401018No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGG175480.10430279956250836No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGAC169310.10063543990157447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)