FastQCFastQC Report
Mon 14 Aug 2023
SRR6215554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6215554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29917538
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length35-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1204760.4026935638888467No Hit
GTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGT921800.3081135887585402No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG832380.2782247656876044No Hit
GTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCA823670.2753134298684604No Hit
CTCCTATTAGATTGATTGAAGGGGGTAGAGCTAGATTAGCTAGACTTGCT793000.26506191786235883No Hit
GCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGG710250.23740255631997526No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC683720.22853484802125096No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGAT622150.20795494602530462No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC609720.20380019238214053No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA587490.19636976812731047No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC541480.18099082885764195No Hit
CTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGC539060.1801819387678224No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG523100.17484727520025212No Hit
CTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGA523010.1748171925109613No Hit
ATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGG517640.17302225871660964No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA504740.16871040658492686No Hit
GGTAGCTGTTGGTGGGCTAATATTTATTAATACTAGAGTAGCTCCTCCGA487180.16284093965218663No Hit
TGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTT473700.15833522130062977No Hit
CTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCAT466020.15576816514781397No Hit
GCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCA459050.15343842798829235No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG458880.15338160513074303No Hit
CATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGG435680.14562695633577868No Hit
GGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTCCA426290.14248832908643752No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA413340.1381597643495932No Hit
GGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCA410350.13716035056093187No Hit
CTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGG405540.13555259794438967No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG401570.13422561709456174No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG399600.13356714045119622No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG364520.12184157666984495No Hit
GTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAG359780.12025722170052897No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG353330.11810129563468758No Hit
GGGTGTTCTACTGGTTGGCCCCCAATTCAGGTTAAGATAAGTAGGTTGGC350500.11715536218254324No Hit
CAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATA338710.11321452988544713No Hit
GTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACAC333730.11154995441135564No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA328200.10970154028048698No Hit
GGTATATCAAAGAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATC323450.10811384279013868No Hit
GTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGAT317630.10616849554933297No Hit
GCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAAT312720.10452731772246768No Hit
TGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAA310970.10394237654181304No Hit
GGGTATATCAAAGAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT304800.1018800410648764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)