FastQCFastQC Report
Tue 15 Aug 2023
SRR6215556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6215556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44190000
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length35-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGT2007800.4543561891830731No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG1913760.4330753564154786No Hit
CCCATGGATAAAGTACAAGCTGGAACCATTGGAAGAACATGTGTTGGGGC1469100.3324507807196198No Hit
GCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCA1014330.22953835709436524No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC923730.20903598099117449No Hit
GTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCA892440.2019551934826884No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG843450.19086897488119484No Hit
CTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCAT839160.18989816700610998No Hit
CCATGGATAAAGTACAAGCTGGAACCATTGGAAGAACATGTGTTGGGGCC807400.18271102059289432No Hit
GTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACAC799090.18083050463905861No Hit
GTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAG796620.1802715546503734No Hit
CTCCGCTCCTAAGTATTTTTCAGGTGTGGTACCCTCTGGAAGTTTAACGA742950.16812627291242363No Hit
CATGGATAAAGTACAAGCTGGAACCATTGGAAGAACATGTGTTGGGGCCA736770.1667277664630007No Hit
GTGGTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGT651170.14735686806969903No Hit
CTTCGGTGTTATCCAGGACAGTCTGGTGTTTCACAAAGGCTACATCTCCT641690.14521158633174927No Hit
CTCCTAAGTATTTTTCAGGTGTGGTACCCTCTGGAAGTTTAACGAAACAT617810.13980764878931887No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC544690.12326091875990042No Hit
CCCCTCTTCCATGGCTCCCAGCCTGCCCTGCCATGTGCAGCCTTCTAGTG494550.11191446028513238No Hit
GCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGG487170.11024439918533606No Hit
GTCCGCTTCTCCGTTCACAATCTTTTCAATGCAGTCCTCAGTGGTCTCTG483150.1093346911065852No Hit
GTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTTATG462870.10474541751527494No Hit
GGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTCCA460530.10421588594704684No Hit
GGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAG459160.10390586105453724No Hit
GTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGATCGTAACGGAAGCGT448210.10142792486988006No Hit
GGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGATCGTAACGGAAG441950.1000113147770989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)