FastQCFastQC Report
Mon 14 Aug 2023
SRR6215568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6215568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45733622
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length35-100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG2312880.5057285862904102No Hit
CCCATGGATAAAGTACAAGCTGGAACCATTGGAAGAACATGTGTTGGGGC2309890.505074800329613No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG1063060.23244605467723506No Hit
CCATGGATAAAGTACAAGCTGGAACCATTGGAAGAACATGTGTTGGGGCC989480.21635723494631587No Hit
CATGGATAAAGTACAAGCTGGAACCATTGGAAGAACATGTGTTGGGGCCA972100.2125569673882379No Hit
GTGGTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGT924530.2021554295437173No Hit
CTTCGGTGTTATCCAGGACAGTCTGGTGTTTCACAAAGGCTACATCTCCT899850.19675896214824184No Hit
CTCCTAAGTATTTTTCAGGTGTGGTACCCTCTGGAAGTTTAACGAAACAT823170.1799923041302086No Hit
CTCCTCCATGTGCTCACGCACCTCCTCTAGGCGGTCACGGGCCTGGTTGC818750.1790258379272912No Hit
CTCCGCTCCTAAGTATTTTTCAGGTGTGGTACCCTCTGGAAGTTTAACGA750780.1641636868385364No Hit
GGGGACATATGTTTCATCAACTGTCAGAGCAGAGAAGCATGGCCGCCTTT749430.16386849919737387No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG719700.15736781136643846No Hit
CCTCCATCTTGGAGCGCACCTCCTCCATGTGCTCACGCACCTCCTCTAGG692370.1513919015642365No Hit
GTCCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCT671480.14682414613913589No Hit
CTCCCCTTTAAAGAACAGCATGGTCCCATTGTGATCCATGGTCGCAGCAT627240.13715073780948292No Hit
CCCCTCTTCCATGGCTCCCAGCCTGCCCTGCCATGTGCAGCCTTCTAGTG606130.13253487773174844No Hit
GTCCGCTTCTCCGTTCACAATCTTTTCAATGCAGTCCTCAGTGGTCTCTG594960.13009247332301824No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG575220.12577617403668576No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA565620.123677061921752No Hit
GTGCTATTTCCATGAGCAGTTCCATTTCTGGGTCTACCATGGCCTCTGCC563340.12317852279445525No Hit
CCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCT555940.1215604572058605No Hit
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG546110.11941105386317315No Hit
GGGGTCTTCTCATGCAGCAGACACACACGGTTCAGGATTGCAGACAGATA538770.11780610772529673No Hit
CCCCTGCCTTGTACACAGCTAGGCGCTTCTGCAGATCATCGGCATCCCGC531870.1162973708926881No Hit
GGCCGCCTTTCCACCAGGGATCCACTACAGCACTTGGTAACATGCTCACT519760.11364942842270398No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT506680.11078938816610677No Hit
CGGGAGGATTGGCTTCAGCGCAGCACTTTTCCAGAGTGGCTTCATATTTC505220.11047014819862726No Hit
CTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCTT498420.10898327711721587No Hit
CTCGGAGAATCTTTATTAAGCAAGGGCCACCAGAGGGCCAGAGAGGTGCT486640.10640749162618258No Hit
CTCTGATCTTCAGGAAGTGTACAACACTTGGTGCCCACTCTTCCTAGGTT483310.10567936211131496No Hit
GTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGAC483060.10562469773332189No Hit
CTCCATCTTGGAGCGCACCTCCTCCATGTGCTCACGCACCTCCTCTAGGC471880.10318010674947198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)