FastQCFastQC Report
Fri 7 Jul 2023
SRR6240171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6240171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28446082
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length43
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGTCACTTCCAGCGGTCGTATGCCGTCTTCTGCTTGAA26481489.309359369771908Illumina Single End Adapter 2 (95% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGTGTCAGTCA21383197.517094972868319No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGTCAGTC19580476.883362707032906No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGTGTCAGTCAC12646114.445642109869471No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTTC8400422.953102645207871No Hit
GGAGGACGATGCGGATCACGGGTGTCAGTCACTTCCAGCGGTC7235522.5435910646675346No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTCC6931942.4368698648903564No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTTCC5799382.0387271610902338No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTCCA5429711.90877253324377No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCGTGTCAGTCACT5028591.7677619012699184No Hit
GGGAGGACGATGCGGATCACGGGTGTCAGTCACTTCCAGCGGT4925051.7313632155036323No Hit
GAATACAAGCTTGGGCTGCAGGTCGAGTGTCAGTCACTTCCAG4630361.627767226432097No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGTGTCAGTCAC4582961.6111041232321555No Hit
AGGGAGGACGATGCGGATCACGGGTGTCAGTCACTTCCAGCGG4034251.4182093688684438No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGTCAGT2995271.0529639899090497No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCGTGTCAGTCACTT2993391.0523030904572377No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGTGTCAGTCACT2978721.047145965479534No Hit
GAATACAAGCTTGGGCTGCAGGTCGGTGTCAGTCACTTCCAGC2735100.9615032397080203No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGTCAGTCACT2723320.957362071866347No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCGTGTCAGTCACTT2439810.8576963252795236No Hit
GAATACAAGCTTGGGCTGCAGGTCGACGTGTCAGTCACTTCCA2429110.8539348230803807No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGTCAGTCACTT2233190.7850606631872888No Hit
GGACGATGCGGATCACGGGTGTCAGTCACTTCCAGCGGTCGTA2141990.7530000089291735No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGTCAGTCACT2105830.74028824074964No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCGTGTCAGTCACTTCC2039750.7170583281029704No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATGTGTCAGTCA1914800.6731331225157826No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCGTGTCAGTCACTTC1652110.5807864858155158No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGGTGTCAGTCACTT1385330.4870020412652962No Hit
GTGTCAGTCACTTCCAGCAGGGAGGACGATGCGGATCACGGGT1255400.44132615521532986No Hit
GACGATGCGGATCACGGGTGTCAGTCACTTCCAGCGGTCGTAT986670.34685620325498606No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTC967770.3402120545107056No Hit
AGGACGATGCGGATCACGGGTGTCAGTCACTTCCAGCGGTCGT963810.33881994715476105No Hit
GTGTCAGTCACTTCCAGCGGAGGGAGGACGATGCGGATCACGG963510.33871448447628044No Hit
TGTCAGTCACTTCCAGCGGTCGTATGCCGTCTTCTGCTTGAAA962130.3382293561552695Illumina Single End Adapter 2 (95% over 23bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGGTGTCAGTCAC949400.3337542231650742No Hit
GAGGACGATGCGGATCACGGGTGTCAGTCACTTCCAGCGGTCG916970.3223537076213167No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCACT908530.3193866909333946No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGGTGTCAGTCACTT864870.30403835579184507No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGTCAGTCA827550.2909187985888531No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGGTGTCAGTCACT797270.28027409890754024No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGTGTCAGTCA788230.2770961568626569No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCCGAGTGTCAGTCAC715340.2514722414144767No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTT715250.2514406026109325No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATGTGTCAGTC698080.2454046219792237No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTT644970.22673421246553394No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCAC637820.2242206852950786No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTCAGTCACTTC616310.21665901124801654No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGTGTCAGTCA594900.20913249142711465No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGTGTCAGTC546270.19203699124540244No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGTGTCAGTCAC527140.18531198778095345No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGTCAGTCACTTCCAG471150.1656291365538495No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCGTGTCAGTCA471140.16562562113123347No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGTGTCAGTC460010.16171295575960162No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGTGTCAGTCA446070.15681245663286775No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGGTGTCAGTCA426490.14992925915069782No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGTGTCAGTCACTTCCA411720.14473697994683415No Hit
GAATACAAGCTTGGGCTGCAGGTCGTGTCAGTCACTTCCAGCG410520.1443151292329116No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACT396120.13925292066584075No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGTGTCAGTCACT381310.134046579771513No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGTGTCAGTCA370210.1301444606677292No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGTGTCAGTCA367930.12934294431127633No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGTGTCAGTCAC366620.12888242394857752No Hit
GGCCGCCGGTGAAATACCACTACTCTCATGTGTCAGTCACTTC351730.12364795967332162No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGTGTCAGTCA348930.12266364134083563No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGTGTCAGTC347350.12210820456750424No Hit
ACATATACTAAAATTGGAACGATACAGAGAAGTGTCAGTCACT343380.12071258178894373No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGTCAGTCACTTCCAG339760.11943999880194398No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGGTGTCAGTCACTTC338550.11901463266540538No Hit
GTGTCAGTCACTTCCAGCGGGGAGGACGATGCGGATCACGGGT337880.11877909935013194No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGTGTCAGTC313900.11034911591691257No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGTCAGTCACTTCCA305330.1073363987349822No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAGTGTCAGTCACTTCC302020.10617279384907911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)