FastQCFastQC Report
Tue 4 Jul 2023
SRR6240172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6240172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27861409
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length44
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTCCAG19447576.980110015254433No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTCCA15948535.724236703176067No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTTCCA10497083.7676055794593877No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGTCAGTCACTT9947633.5703973191018443No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTTCC9200043.3020727702608292No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGTGTCAGTCAC9013623.235163017060623No Hit
GTGTCAGTCACTTCCAGCGGTCGTATGCCGTCTTCTGCTTGAAA7660332.7494409920187457Illumina Single End Adapter 2 (95% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCACTT7471552.681684189051602No Hit
GAATACAAGCTTGGGCTGCAGGTCGAGTGTCAGTCACTTCCAGC7250972.6025137493943684No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGTGTCAGTCAC7032022.5239283483473502No Hit
GAATACAAGCTTGGGCTGCAGGTCGGTGTCAGTCACTTCCAGCG6723052.413033023563166No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGTGTCAGTCACT5228141.8764808341171835No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCGTGTCAGTCACTTC5116571.8364361974658208No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGGTGTCAGTCACTTC4945161.7749138243510945No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGTGTCAGTCACT4739131.7009656618586662No Hit
GAATACAAGCTTGGGCTGCAGGTCGACGTGTCAGTCACTTCCAG4138781.4854884044091239No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCACT3727491.3378684473567004No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCACTTC3408201.2232690744391284No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCGTGTCAGTCACTTCCA3175551.1397664777111596No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCGTGTCAGTCACTT2883211.0348399824287422No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGTGTCAGTCACT2513220.902043396297725No Hit
GAGGACGATGCGGCAGATCGTGTCAGTCACTTCCAGCGGTCGTA2399790.86133116957581No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGTCAGTCACTTC2322430.8335651653511134No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGTGTCAGTCACTT2000790.7181223318605315No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTCAGTCACTTCC1622620.5823897850966547No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGTCAGTCACTT1456610.5228055767028869No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCGTGTCAGTCACTTCC1425370.5115929348727482No Hit
AGGACGATGCGGCAGATCGTGTCAGTCACTTCCAGCGGTCGTAT1295290.4649047002612107No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGTGTCAGTCAC1266970.454740103057961No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGTCAGTCA1160090.41637879835869035No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGTGTCAGTCA1116870.40086630220316566No Hit
GTGTCAGTCACTTCCAGCGGAGGGAGGACGATGCGGCAGATCGT1071100.3844385615960772No Hit
GGAGGACGATGCGGCAGATCGTGTCAGTCACTTCCAGCGGTCGT1049660.3767433298150858No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTCC1043080.3743816402106584No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGTCAGTC1007160.3614892556223557No Hit
CGCGGCGTGGGAAATGTGGCGTACGGTGTCAGTCACTTCCAGCG854870.3068294212973938No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTC797980.2864104970427016No Hit
GAATACAAGCTTGGGCTGCAGGTCGTGTCAGTCACTTCCAGCGG784520.2815794420160158No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGTGTCAGTCACTTCCAG702350.2520870355120949No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCACTTCC697890.25048625502034016No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCGTGTCAGTCACTTC694910.24941667522988517No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGGTGTCAGTCACT658970.23651711225372699No Hit
GTGTCAGTCACTTCCAGCGGGTGTCAGTCACTTCCAGCGGGTGT654930.23506707790693573No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGTGTCAGTCACTTCCA602830.21636737754361238No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGTGTCAGTCAC569350.20435075627366872No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTTC552590.19833526725084147No Hit
GGGAGGACGATGCGGCAGATCGTGTCAGTCACTTCCAGCGGTCG522160.18741335012884666No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGTGTCAGTCACTTCCAGCG515800.1851306227908287No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGTGTCAGT481810.1729309526305723No Hit
GTGTCAGTCACTTCCAGCGGGTGTCAGTCACTTCCAGCGGTCGT463570.16638426290644526No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGTCAGTCACTTCCAGC451530.16206287341749298No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGTGTCAGTCACT449400.16129837511089262No Hit
GGACGATGCGGCAGATCGTGTCAGTCACTTCCAGCGGTCGTATG442100.15867826354367076No Hit
GAATACAAGCTTGGGCTGCAGGTCGAAGGTGTCAGTCACTTCCA435630.15635605507244807No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCGAGTGTCAGTCAC396980.14248382054188286No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGTGTCAGTCA380460.13655447217332045No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTT367750.1319926066912122No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGGTGTCAGTCAC364960.13099122158538357No Hit
GAGGGCGCACCACCGGCCCGTCTCGCGTGTCAGTCACTTCCAGC362550.13012622584880756No Hit
TCGCGGCGTGGGAAATGTGGCGTACGTGTCAGTCACTTCCAGCG343560.12331034658010297No Hit
TTTCGCCGAATCCCGGGGCCGCGGAAGGTGTCAGTCACTTCCAG341180.12245611842531007No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGTGTCAGTCA337820.12125014926560246No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTGTGTCAGTCACTTC335400.12038156433509878No Hit
GACGATGCGGCAGATCGTGTCAGTCACTTCCAGCGGTCGTATGC330640.118673108025513No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTGTGTCAGTCACTTC307280.11028875101040296No Hit
GAATACAAGCTTGGGCTGCAGGTCGGTGTCCGTCACTTCCAGCG302780.10867361374293741No Hit
GAATACAAGCTTGGGCTGCAGGAGGGTGTCAGTCACTTCCAGCG301330.10815318062342073No Hit
CGTCGCGGCGTGGGAAATGTGGCGTAGTGTCAGTCACTTCCAGC290600.10430197553899732No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCGTGTCAGTCACTTCCAG289920.10405791035191364No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTGTGTCAGTCACTTCC282510.10139831765148705No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)