FastQCFastQC Report
Tue 4 Jul 2023
SRR6240173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6240173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29195323
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length43
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTCCA17046445.838757118734394No Hit
GTGTCAGTCACTTCCAGCGGTCGTATGCCGTCTTCTGCTTGAA15598505.342807818909899Illumina Single End Adapter 2 (95% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTCC13918814.7674793664725No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTTC12687084.345586448897996No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCGTGTCAGTCACTTCC12136724.1570768030208125No Hit
GAATACAAGCTTGGGCTGCAGGTCGAGTGTCAGTCACTTCCAG9751273.3400110010771247No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGTCAGTCACT9662493.309602020844229No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGTGTCAGTCA9342083.1998549904722755No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGTGTCAGTCA8560352.9320963498160304No Hit
GAATACAAGCTTGGGCTGCAGGTCGGTGTCAGTCACTTCCAGC8557202.9310174098776027No Hit
GAATACAAGCTTGGGCTGCAGGTCGACGTGTCAGTCACTTCCA7060012.4181989697459416No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGTGTCAGTCAC5874122.0120071971801785No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCACT5274111.8064914027496801No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGGTGTCAGTCACTT4735761.6220954294631371No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGTGTCAGTCAC4088531.4004058115746827No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCGTGTCAGTCACTTCC3664761.2552558503976818No Hit
GGAGGACGATGCGGTTAGGCGGTGTCAGTCACTTCCAGCGGTC3435771.1768220546832107No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGTGTCAGTCAC2710240.9283130726109794No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGTCAGTCACTT2552560.8743044219788217No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCGTGTCAGTCACTT2277450.780073575483306No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGTCAGTCACT2214370.7584673750655199No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCACTT2194350.7516101123457343No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCGTGTCAGTCACTTC2158110.7391971652445839No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTCAGTCACTTC1848490.6331459323125146No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGTGTCAGTCACT1817530.6225414940605384No Hit
AGGGAGGACGATGCGGTTAGGCGGTGTCAGTCACTTCCAGCGG1797550.6156979321653677No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCAC1761790.6034493949595968No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTGTGTCAGTCA1457500.49922379690747043No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTGTGTCAGTCAC1439430.4930344493876639No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGTGTCAGTCA1413200.4840501336464063No Hit
GAATACAAGCTTGGGCTGCAGGTCGTGTCAGTCACTTCCAGCG1411300.4833993444771959No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGTCAGTC1163820.39863234258446123No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGTGTCAG1140950.39079889611085994No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGTCAGT872480.29884238650142697No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGTGTCAGTC862010.2952561956584622No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGTGTCAGTC841910.28837153128944665No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTGTGTCAGTCACTT714640.24477893257080938No Hit
GGGAGGACGATGCGGTTAGGCGGTGTCAGTCACTTCCAGCGGT653140.22371391472531407No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGTGTCAGTC584040.20004574020297702No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCGTGTCAGTCACTT519810.17804564107751095No Hit
GAGGACGATGCGGTTAGGCGGTGTCAGTCACTTCCAGCGGTCG506580.17351409333611414No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTGTGTCAGTCACTTC486860.1667595868009407No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTTC466320.1597242133611606No Hit
GAATACAAGCTTGGGCTGCAGGTCGAAGGTGTCAGTCACTTCC463970.15891928991503193No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTGTGTCAGTC459190.15728204137354468No Hit
GAATACAAGCTTGGGCTGCAGGAGGGTGTCAGTCACTTCCAGC458510.15704912735509038No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGTGTCAGTCACTTCCAG449270.15388423686903552No Hit
GAATACAAGCTTGGGCTGCAGGTCGAAGTGTCAGTCACTTCCA435830.14928075979841018No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGGTGTCAGTCA423050.14490334633393162No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGGTGTCAGT419340.14363259485089444No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCGTGTCAGTCACT403500.13820706830337176No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCGTGTCAGT384010.13153134150973428No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGTGTCAGTCACTTCC356970.12226958407002382No Hit
ACATATACTAAAATTGGAACGATACAGAGAAGTGTCAGTCACT349640.11975890795933308No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGGTGTCAGTCACTT335860.11503897387948063No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCCGAGTGTCAGTCAC323410.11077459221807548No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGGTGTCAGTCA318660.10914761929504942No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGTGTCAGTCACTTCC300950.1030815791967775No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGTGTCAGTCACTTCCA299180.10247531770756568No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGTCAGTCACTTCCA292310.10012220107994696No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)